Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6777 |
| PDBID: | 4C2I |
| Chains: | MN_A |
| Organism: | dengue virus type 1, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 6.00 |
| Reference: | 10.1002/emmm.201303404 |
| Antibody | |
| Antibody: | 1F4 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Dengue virus (DENV) type 1 envelope glycoprotein |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: M
Mutation: NULL
| >4C2I_M|Chain G[auth H], I[auth M]|HEAVY CHAIN FAB FRAGMENT OF ANTIBODY 1F4|HOMO SAPIENS (9606) GSWAQVQLVESGGGVVQPGRSLRLSCAASGFTFSAYGMHWVRQAPGKGLEWVAIIWYDGSNKYYADSVKGRFTISRDNSKNTLHLQMNSLRAEDTAIYYCARDKNPGTKPYYHYGMDVWGQGTTVTVSSAGTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSC |
Light Chain: N
Mutation: NULL
| >4C2I_N|Chain H[auth L], J[auth N]|LIGHT CHAIN FAB FRAGMENT OF ANTIBODY 1F4|HOMO SAPIENS (9606) LATMAWMMLLLLTLITHCAGSWAQSVLTQPPSVSEAPRQRVTISCSGSSSNIGNNAVNWYQQFPGKAPKLLIYYDDLLPSGVSDRFSGSKSGTSASLAISGLQSEDEAYYYCAAWDDSLIGVVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: A
Mutation: NULL
| >4C2I_A|Chain A, C, E|ENVELOPE PROTEIN|DENGUE VIRUS 1 (11053) MRCVGISNRDFVEGLSATGWVDVVLEHGSCVTTMAKDKPTLDIELLKTEVTNPAVLRKLCIEAKISNTTTDSRCPTQGEATLVEEQDANFVCRRTFVDRGWGNGCGLFGKGSLITCAKFKCVTKLEGKIVQYENLKYSVIVTVHTGDQHQVGNESTEHGTTATITPQAPTTEIQLTDYGALTLDCSPRTGLDFNEMVLLTMKEKSWLVHKQWFLDLPLPWTSGASTSQETWNRQDLLVTFKTAHAKKQEVVVLGSQEGAMHTALTGATEIQTSGTTTIFAGHLKCRLKMDKLTLKGMSYVMCTGSFKLEKEVAETQHGTVLVQIKYEGTDAPCKIPFSTQDEKGVTQNGRLITANPIVTDKEKPVNIEAEPPFGESYIVIGAGEKALKLSWFKKGSSIGKMFEATARGARRMAILGDTAWDFGSIGGVFTSVGKLVHQIFGTAYGVLFSGVSWTMKIGIGVLLTWLGLNSRSTSLSMTCIAVGLVTLYLGVMVQA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition M: ALA35 LYS109 PRO110 TYR111 TYR112 N: GLY53 LEU78 PRO79 SER80 SER89 LYS90 GLY92 A: THR51 ASN52 SER155 THR156 ALA162 THR163 ILE164 THR165 ALA168 PRO169 THR171 THR176 GLY274 THR275 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
