Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6765 |
| PDBID: | 4AEI |
| Chains: | HL_A |
| Organism: | Androctonus australis hector, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.30 |
| Reference: | 10.1074/jbc.M111.315382 |
| Antibody | |
| Antibody: | 4C1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Androctonus australis hector Ⅱ (AahⅡ) toxin |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >4AEI_H|Chain D[auth H], E[auth I], F[auth J]|FAB ANTIBODY HEAVY CHAIN|MUS MUSCULUS (10090) EVHLVESGGGLVKPGGSLKLSCAASGFTFSGYYMYWVRQTPEKRLEWVASISDGGSFTYYPDSVKGRFTISRDNAKNNLYLQMSSLRSDDTAMYYCSRPDDYSYDGFAYWGQGTLVTVSAAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDCGCKPCIC |
Light Chain: L
Mutation: NULL
| >4AEI_L|Chain G[auth L], H[auth M], I[auth N]|FAB ANTIBODY LIGHT CHAIN|MUS MUSCULUS (10090) DVLMTQSPLSLPVSLGDQASISCRSSQSIVHSNGNTYLEWYLQKPGQSPNLLIYKVSNRFSGVPDRFSGSGSGTDFTLKISRVEAEDLGVYYCFQGSHVPLTFGAGTKLELKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: A
Mutation: NULL
| >4AEI_A|Chain A, B, C|ALPHA-MAMMAL TOXIN AAH2|ANDROCTONUS AUSTRALIS HECTOR (70175) VKDGYIVDDVNCTYFCGRNAYCNEECTKLKGESGYCQWASPYGNACYCYKLPDHVRTKGPGRCHX |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition H: GLY31 TYR32 TYR33 TYR35 TRP47 SER50 ASP53 PHE57 TYR59 PRO99 TYR102 SER103 TYR104 ASP105 GLY106 PHE107 L: HIS31 SER32 ASN33 GLY34 ASN35 TYR37 GLU39 LYS55 PHE94 GLY96 SER97 HIS98 VAL99 LEU101 A: ASP8 ASP9 VAL10 ASN11 CYS12 THR13 TYR14 PHE15 PRO41 TYR42 GLY43 ARG56 LYS58 GLY59 PRO60 GLY61 ARG62 CYS63 HIS64 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
