Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 676 |
| PDBID: | 3UZE |
| Chains: | A_C |
| Organism: | Mus musculus, dengue virus type 3 |
| Method: | XRD |
| Resolution (Å): | 2.04 |
| Reference: | 10.1016/j.str.2012.01.001 |
| Antibody | |
| Antibody: | 4E11 scFv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | dengue virus serotype 3 envelope protein domain III |
| Antigen mutation: | No |
| Durg Target: | P27915 |
Sequence information
Antibody
Chain: A
Mutation: NULL
| >3UZE_A|Chain A, B|Variable domains of murine anti-dengue Mab 4E11|Mus musculus (10090) EVKLLEQSGAELVKPGASVRLSCTASGFNIKDTYMSWVKQRPEQGLEWIGRIDPANGDTKYDPKFQGKATITADTSSNTAYLHLSSLTSGDTAVYYCSRGWEGFAYWGQGTLVTVSAGGGGSGGGGSGGGGSELVMTQTPASLAVSLGQRATISCRASENVDRYGNSFMHWYQQKAGQPPKLLIYRASNLESGIPARFSGSGSRTDFTLTINPVEADDVATYFCQRSNEVPWTFGGGTKLEIKRPLEHHHHHH |
Antigen
Chain: C
Mutation: NULL
| >3UZE_C|Chain C, D|Envelope protein|Dengue virus 3 (11069) KGMSYAMCLNTFVLKKEVSETQHGTILIKVEYKGEDAPCKIPFSTEDGQGKAHNGRLITANPVVTKKEEPVNIEAEPPFGESNIVIGIGDKALKINWYRKGPFEDDDDKAGWSHPQFEKGGGSGGGSGGGSWSHPQFEK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition A: LYS3 LYS31 ASP32 THR33 TYR34 ASP53 ALA55 ARG99 TRP101 GLU102 ALA105 TYR106 ARG163 TYR164 GLY165 ASN166 PHE168 LEU182 TYR185 ARG186 ASN189 GLU191 SER192 C: THR305 PHE306 VAL307 LEU308 LYS309 LYS310 GLU311 VAL312 LYS323 GLU325 LYS327 GLU329 LYS361 ASP384 LYS385 LEU387 LYS388 ILE389 ASN390 TRP391 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
