Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 675 |
| PDBID: | 3UYP |
| Chains: | A_B |
| Organism: | Mus musculus, dengue virus type 4 |
| Method: | XRD |
| Resolution (Å): | 2.00 |
| Reference: | 10.1016/j.str.2012.01.001 |
| Antibody | |
| Antibody: | 4E11 scFv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | dengue virus serotype 4 envelope protein domain III |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: A
Mutation: NULL
| >3UYP_A|Chain A|anti-dengue Mab 4E11|Mus musculus (10090) EVKLLEQSGAELVKPGASVRLSCTASGFNIKDTYMSWVKQRPEQGLEWIGRIDPANGDTKYDPKFQGKATITADTSSNTAYLHLSSLTSGDTAVYYCSRGWEGFAYWGQGTLVTVSAGGGGSGGGGSGGGGSELVMTQTPASLAVSLGQRATISCRASENVDRYGNSFMHWYQQKAGQPPKLLIYRASNLESGIPARFSGSGSRTDFTLTINPVEADDVATYFCQRSNEVPWTFGGGTKLEIKRPLEHHHHHH |
Antigen
Chain: B
Mutation: NULL
| >3UYP_B|Chain B|Envelope protein|Dengue virus 4 (11070) MGMSYTMCSGKFSIDKEMAETQHGTTVVKVKYEGAGAPCKVPIEIRDVNKEKVVGRIISSTPFAENTNSVTNIELEPPFGDSYIVIGVGDSALTLHWFRKGSSIGKLEHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition A: VAL2 LEU4 GLY27 PHE28 LYS31 ASP32 THR33 TYR34 ASP53 ALA55 ARG99 GLY100 TRP101 GLU102 ALA105 TYR106 ARG163 TYR164 GLY165 ASN166 PHE168 TYR185 ARG186 ASN189 LEU190 GLU191 SER192 GLU229 B: LYS305 PHE306 SER307 ILE308 ASP309 LYS310 GLU311 MET312 LYS323 LYS325 GLU327 ASN360 ASN362 SER363 VAL364 ASN366 SER385 LEU387 THR388 LEU389 HIS390 TRP391 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
