Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6727 |
| PDBID: | 3WFE |
| Chains: | HL_C |
| Organism: | Mus musculus, Pseudomonas aeruginosa PAO1 |
| Method: | XRD |
| Resolution (Å): | 2.49 |
| Reference: | 10.1002/prot.24492 |
| Antibody | |
| Antibody: | 3O0R Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Nitric oxide reductase (NOR) subunit C |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >3WFE_H|Chain B[auth H]|antibody fab fragment heavy chain|Mus musculus (10090) EVQLQQSGTVLARPGASVKMSCKASGYSFTSYWMHWVKQRPGQGLEWIGAVYPGNSDTSYNQKFKGKAKLTAVTSASTAYMELSSLTNEDSAVYYCSRSSLDGYYVKNWCFDVWGQGTTVTVSSAKTTAPSVYPLAPVCGDTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTRPSQSITCNVAHPASSTKVDKKIEPRG |
Light Chain: L
Mutation: NULL
| >3WFE_L|Chain A[auth L]|antibody fab fragment light chain|Mus musculus (10090) DIQMTQSPPYLAASPGETITINCRASKSIRKYLAWYQEKPGKTNKLLIYSGSTLQFGIPSRFSGSGSGTEFTLTISSLEPEDFAMYYCQQHNEYPLTFGAGTKLELKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNE |
Antigen
Chain: C
Mutation: NULL
| >3WFE_C|Chain D[auth C]|Nitric oxide reductase subunit C|Pseudomonas aeruginosa (208964) MSETFTKGMARNIYFGGSVFFILLFLALTYHTEKTLPERTNEAAMSAAVVRGKLVWEQNNCVGCHTLLGEGAYFAPELGNVVGRRGGEEGFNTFLQAWMKIQPLNVPGRRAMPQFHLSEGQVDDLAEFLKWSSKIDTNQWPPNKEG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: ARG30 LYS31 TYR32 TYR49 SER50 THR53 LEU54 PHE56 GLY57 H: GLY26 TYR27 SER28 LEU101 ASP102 TYR105 VAL106 TRP109 C: ARG85 THR93 PHE94 ALA97 TRP98 ILE101 LEU104 ASN105 VAL106 PRO107 ARG109 LYS144 GLU145 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
