Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6715 |
| PDBID: | 3VI4 |
| Chains: | FE_B |
| Organism: | Homo sapiens, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.90 |
| Reference: | 10.1083/jcb.201111077 |
| Antibody | |
| Antibody: | SG/19 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Integrin beta-1 |
| Antigen mutation: | No |
| Durg Target: | P05556 |
Sequence information
Antibody
Heavy Chain: F
Mutation: NULL
| >3VI4_F|Chain F, H|SG/19 Fab fragment (Heavy chain)|Mus musculus (10090) QVHLQQSGAELMKPGASVKISCKATGYTFTSYWIEWVKQRPGHGLEWLGEILPGSGYIHYNEKFKGKATFTTDTSSNTAYMQLSSLTSEDSAVYYCSRALALYAMDYWGQGTSVTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPR |
Light Chain: E
Mutation: NULL
| >3VI4_E|Chain E, G[auth L]|SG/19 Fab fragment (Light chain)|Mus musculus (10090) DIVMTQATPSIPVTPGESVSISCRSNKSLLHSNGNTYLYWFLQRPGQSPRLLIFRMSNLASGVPDRFSGSGSGTAFTLRISRVEAADVGIYFCLQHLEYPFTFGAGTKLELKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: B
Mutation: NULL
| >3VI4_B|Chain B, D|Integrin beta-1|Homo sapiens (9606) QTDENRCLKANAKSCGECIQAGPNCGWCTNSTFLQEGMPTSARCDDLEALKKKGCPPDDIENPRGSKDIKKNKNVTNRSKGTAEKLKPEDIHQIQPQQLVLRLRSGEPQTFTLKFKRAEDYPIDLYYLMDLSYSMKDDLENVKSLGTDLMNEMRRITSDFRIGFGSFVEKTVMPYISTTPAKLRNPCTSEQNCTTPFSYKNVLSLTNKGEVFNELVGKQRISGNLDSPEGGFDAIMQVAVCGSLIGWRNVTRLLVFSTDAGFHFAGDGKLGGIVLPNDGQCHLENNMYTMSHYYDYPSIAHLVQKLSENNIQTIFAVTEEFQPVYKELKNLIPKSAVGTLSANSSNVIQLIIDAYNSLSSEVILENGKLSEGVTISYKSYCKNGVNGTGENGRKCSNISIGDEVQFEISITSNKCPKKDSDSFKIRPLGFTEEVEVILQYICECEGGLENLYFQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| E: HIS31 SER32 ASN33 ASN35 TYR37 PHE54 ARG55 SER57 ASN58 LEU59 SER61 HIS96 LEU97 TYR99 PHE101 F: THR28 THR30 SER31 TYR32 TRP33 GLU50 LEU52 TYR57 HIS59 ALA99 LEU100 ALA101 LEU102 TYR103 B: ASN77 ARG78 SER79 LYS80 GLY81 THR82 ALA83 GLU84 LYS85 LYS87 GLU119 ASP120 ASN151 ARG154 ARG155 ILE156 THR157 SER158 LYS382 SER399 ILE400 GLY401 ASP402 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
