Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6709 |
| PDBID: | 3V6Z |
| Chains: | AB_F |
| Organism: | Mus musculus, Hepatitis B virus |
| Method: | XRD |
| Resolution (Å): | 3.34 |
| Reference: | 10.1016/j.str.2012.10.017 |
| Antibody | |
| Antibody: | e6 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Chronic hepatitis B virus e-antigen (HBeAg) |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >3V6Z_A|Chain A, C|Fab e6 Heavy Chain|Mus musculus (10090) EVQLVESGGDLVKPGGSLKLSCAASGFTFSSYGMSWVRQTPDKRLEWVATISSGGNYIYYPDTVKGRFTISRDNAKNTLYLQMSSLKSEDTAMYYCTREGAYSGSSSYPMDYWGQGTSVTVSSAKTTPPSVYPLAPGCGDTTGSSVTLGCLVKGYFPESVTVTWNSGSLSSSVHTFPALLQSGLYTMSSSVTVPSSTWPSQTVTCSVAHPASSTTVDKKLEPSG |
Light Chain: B
Mutation: NULL
| >3V6Z_B|Chain B, D|Fab e6 Light Chain|Mus musculus (10090) NIMMTQSPSSLAVSAGEKVTMNCKSSQSVLYSSNQKNYLAWYQQKPGQSPKLLIYWASTRESGVPDRFTGSGSGTDFTLTISSVQTEDLAVYYCHQYLSSYMYTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRN |
Antigen
Chain: F
Mutation: C58A/E74D/M103V/C117A/G133A
| >3V6Z_F|Chain E, F|e-antigen|Hepatitis B virus (10407) SKLCLGWLWGMDIDPYKEFGATVELLSFLPSDFFPSVRDLLDTAAALYRDALESPEHASPHHTALRQAILCWGDLMTLATWVGTNLEDPASRDLVVSYVNTNVGLKFRQLLWFHISALTFGRETVLEYLVSFAVWIRTPPAYRPPNAPILSTLPETTVV |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: THR28 SER30 SER31 SER52 SER53 GLY54 ASN56 TYR57 TYR59 ALA101 TYR102 SER103 GLY104 SER105 SER106 B: ASN1 ILE2 GLN27 TYR31 ASN34 TYR38 TYR97 LEU98 SER99 SER100 TYR101 MET102 TYR103 F: GLY20 ALA21 THR22 GLU24 LEU25 PHE28 ARG122 GLU123 THR124 LEU126 GLU127 TYR128 VAL130 SER131 ALA133 VAL134 TRP135 ILE136 ARG137 THR138 PRO139 TYR142 PRO144 PRO148 ILE149 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
