Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6689 |
| PDBID: | 3SQO |
| Chains: | HL_A |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.70 |
| Reference: | 10.1124/jpet.111.187419 |
| Antibody | |
| Antibody: | J16 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | Bococizumab(Phase-III) |
| Antigen | |
| Antigen: | Proprotein convertase substilisin/kexin type 9 (PCSK9) |
| Antigen mutation: | No |
| Durg Target: | Q8NBP7 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >3SQO_H|Chain B[auth H]|J16 Heavy chain|Homo sapiens (9606) QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYYMHWVRQAPGQGLEWMGEISPFGGRTNYNEKFKSRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARERPLYASDLWGQGTTVTVSSASTKGPSVFPLAPSSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSNFGTQTYTCNVDHKPSNTKVDKTVERK |
Light Chain: L
Mutation: NULL
| >3SQO_L|Chain C[auth L]|J16 Light chain|Homo sapiens (9606) DIQMTQSPSSLSASVGDRVTITCRASQGISSALAWYQQKPGKAPKLLIYSASYRYTGVPSRFSGSGSGTDFTFTISSLQPEDIATYYCQQRYSLWRTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGES |
Antigen
Chain: A
Mutation: NULL
| >3SQO_A|Chain A|Proprotein convertase subtilisin/kexin type 9|Homo sapiens (9606) SIPWNLERITPPRYRADEYQPPDGGSLVEVYLLDTSIQSDHREIEGRVMVTDFENVPEEDGTRFHRQASKCDSHGTHLAGVVSGRDAGVAKGASMRSLRVLNCQGKGTVSGTLIGLEFIRKSQLVQPVGPLVVLLPLAGGYSRVLNAACQRLARAGVVLVTAAGNFRDDACLYSPASAPEVITVGATNAQDQPVTLGTLGTNFGRCVDLFAPGEDIIGASSDCSTCFVSQSGTSQAAAHVAGIAAMMLSAEPELTLAELRQRLIHFSAKDVINEAWFPEDQRVLTPNLVAALPPSTHGAGWQLFCRTVWSAHSGPTRMATAIARCAPDEELLSCSSFSRSGKRRGERMEAQGGKLVCRAHNAFGGEGVYAIARCCLLPQANCSVHTAPPAEASMGTRVHCHQQGHVLTGCSSHWEVEDLGTHKPPVLRPRGQPNQCVGHREASIHASCCHAPGLECKVKEHGIPAPQEQVTVACEEGWTLTGCSALPGTSHVLGAYAVDNTCVVRSRDVSTTGSTSEEAVTAVAICCRSRHLAQASQELQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: SER31 TYR32 TYR33 GLU50 GLU95 ARG96 PRO97 LEU98 TYR99 L: SER30 SER31 ALA32 TYR49 SER50 TYR53 ARG54 TYR55 THR56 ARG91 TYR92 LEU94 ARG96 A: SER153 ILE154 PRO155 ARG194 GLU195 GLU197 ARG237 ASP238 ALA239 ASP367 ILE369 ASP374 CYS375 SER376 THR377 CYS378 PHE379 SER381 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
