Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6659 |
| PDBID: | 3PJS |
| Chains: | BA_N |
| Organism: | Mus musculus, Streptomyces lividans |
| Method: | XRD |
| Resolution (Å): | 3.80 |
| Reference: | 10.1073/pnas.1105112108 |
| Antibody | |
| Antibody: | 2 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Voltage-gated potassium channel KcsA |
| Antigen mutation: | Yes |
| Durg Target: | P0A333; P0A334; |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >3PJS_B|Chain B, D|FAB heavy chain|Mus musculus (10090) EVQLVESGGGLVQPGGSLRLSCAASGFNISSYSIHWVRQAPGKGLEWVASISSYYSSTYYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARQPSYHMYSWWVALDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEP |
Light Chain: A
Mutation: NULL
| >3PJS_A|Chain A, C|FAB light chain|Mus musculus (10090) DIQMTQSPSSLSASVGDRVTITCRASQDVNTAVAWYQQKPGKAPKLLIYSASFLESGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQYYSYSAPVTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE |
Antigen
Chain: N
Mutation: H31Q/R123Q/E126Q/R127Q/R128Q/G129Q/H130Q
| >3PJS_N|Chain E[auth K], F[auth L], G[auth M], H[auth N]|Voltage-gated potassium channel|Streptomyces lividans (1916) MHHHHHHPPMLSGLLARLVKLLLGRHGSALQWRAAGAATVLLVIVLLAGSYLAVLAERGAPGAQLITYPRALWWSVETATTVGYGDLYPVTLWGRLVAVVVMVAGITSFGLVTAALATWFVGQEQQQQQQFVRHSEKAAEEAYTRTTRALHERFDRLERMLDDNRR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: ASP28 ASN30 THR31 ARG66 GLY68 TYR92 TYR94 N: HIS145 ASP149 GLU152 ASP156 ASP157 ARG160 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
