Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6656 |
| PDBID: | 3PJS |
| Chains: | DC_K |
| Organism: | Mus musculus, Streptomyces lividans |
| Method: | XRD |
| Resolution (Å): | 3.80 |
| Reference: | 10.1073/pnas.1105112108 |
| Antibody | |
| Antibody: | 2 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Voltage-gated potassium channel KcsA |
| Antigen mutation: | Yes |
| Durg Target: | P0A333; P0A334; |
Sequence information
Antibody
Heavy Chain: D
Mutation: NULL
| >3PJS_D|Chain B, D|FAB heavy chain|Mus musculus (10090) EVQLVESGGGLVQPGGSLRLSCAASGFNISSYSIHWVRQAPGKGLEWVASISSYYSSTYYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARQPSYHMYSWWVALDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEP |
Light Chain: C
Mutation: NULL
| >3PJS_C|Chain A, C|FAB light chain|Mus musculus (10090) DIQMTQSPSSLSASVGDRVTITCRASQDVNTAVAWYQQKPGKAPKLLIYSASFLESGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQYYSYSAPVTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE |
Antigen
Chain: K
Mutation: H31Q/R123Q/E126Q/R127Q/R128Q/G129Q/H130Q
| >3PJS_K|Chain E[auth K], F[auth L], G[auth M], H[auth N]|Voltage-gated potassium channel|Streptomyces lividans (1916) MHHHHHHPPMLSGLLARLVKLLLGRHGSALQWRAAGAATVLLVIVLLAGSYLAVLAERGAPGAQLITYPRALWWSVETATTVGYGDLYPVTLWGRLVAVVVMVAGITSFGLVTAALATWFVGQEQQQQQQFVRHSEKAAEEAYTRTTRALHERFDRLERMLDDNRR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: ASN30 THR31 TYR92 SER93 TYR94 D: SER52 TYR54 TYR55 SER56 SER57 TYR59 TYR102 HIS103 MET104 TYR105 TRP107 TRP108 K: ARG139 ARG142 HIS145 GLU146 ARG147 ASP149 ARG150 ARG153 MET154 ASP157 ASN158 ARG160 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
