Details

Structure visualisation

	Display styles : Spacefill Wire Ball&stick Cartoon	Display color : Default Color by B-factor Color by sec.structure Color by element
Spin on/off

Entry information

Complex
AACDB_ID:	6635
PDBID:	3J6U
Chains:	HL_A
Organism:	dengue virus type 3, Homo sapiens
Method:	EM
Resolution (Å):	9.00
Reference:	10.1038/ncomms7341
Antibody
Antibody:	5J7 Fab
Antibody mutation:	No
INN (Clinical Trial):
Antigen
Antigen:	Dengue virus (DENV) type 3 envelope protein
Antigen mutation:	No
Durg Target:	P27915

Sequence information

Antibody

Heavy Chain: H
Mutation: NULL

>3J6U_H|Chain G[auth H]|Fab 5J7 heavy chain|Homo sapiens (9606)
WVPGSWAQVQLVQSGAELRKPGSSVKVSCRASGGTFSSYTFNWVRQAPGQGLEWMGGFIPVFNTTNYAQTFQGRVTIAADKSTSTAYMELRSLRSEDTAIYYCARDKELLFSRAFDIWGQGTMVTVSSAGTKGPS

Light Chain: L
Mutation: NULL

>3J6U_L|Chain H[auth L]|Fab 5J7 light chain|Homo sapiens (9606)
WVPGVHSDIQMTQSPSSLSASVGDRVTITCRASQSISRYLNWYQREPGKAPKLLIYGASSLQRGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSQYIPYTFGQGTKVDIKRTVA

Antigen

Chain: A
Mutation: NULL

>3J6U_A|Chain A, C, E|envelope protein|Dengue virus 3 (11069)
MRCVGVGNRDFVEGLSGATWVDVVLEHGGCVTTMAKNKPTLDIELQKTEATQLATLRKLCIEGKITNITTDSRCPTQGEAVLPEEQDQNYVCKHTYVDRGWGNGCGLFGKGSLVTCAKFQCLEPIEGKVVQYENLKYTVIITVHTGDQHQVGNETQGVTAEITPQASTTEAILPEYGTLGLECSPRTGLDFNEMILLTMKNKAWMVHRQWFFDLPLPWASGATTETPTWNRKELLVTFKNAHAKKQEVVVLGSQEGAMHTALTGATEIQNSGGTSIFAGHLKCRLKMDKLELKGMSYAMCTNTFVLKKEVSETQHGTILIKVEYKGEDAPCKIPFSTEDGQGKAHNGRLITANPVVTKKEEPVNIEAEPPFGESNIVIGIGDNALKINWYKKGSSIGKMFEATARGARRMAILGDTAWDFGSVGGVLNSLGKMVHQIFGSAYTALFSGVSWVMKIGIGVLLTWIGLNSKNTSMSFSCIAIGIITLYLGAVVQA

Interaction

1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å² were classified as interacting residues.

Interacting residues (ΔSASA based)

H: SER37 VAL61 PHE62 ASN63

A: ARG73 CYS74 GLY104 CYS105 GLY106

2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.

Interacting residues (Atom distance based)

Within the threshold, the structure has no interacting amino acids and cannot be displayed. This may be due to the following reasons:
1. The method used to determine the structure is electron microscopy or nuclear magnetic resonance, which results in too low resolution;
2. the coordinates of some amino acids involved in the interaction in the structure were not detected due to technical limitations. The results are less based on atomic coordinates, but the antibody does target the antigen.

Download

Download sequences
Download structure
Download interacting residues (ΔSASA based)
Download interacting residues (Distance based)