Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6612 |
| PDBID: | 3G9A |
| Chains: | B_A |
| Organism: | Aequorea victoria, Camelus dromedarius |
| Method: | XRD |
| Resolution (Å): | 1.61 |
| Reference: | 10.1038/nsmb.1727 |
| Antibody | |
| Antibody: | 3G9A Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Green fluorescent protein (GFP) |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >3G9A_B|Chain B|Minimizer|Camelus dromedarius (9838) MADVQLQESGGGSVQAGGSLRLSCAASGDTFSSYSMAWFRQAPGKECELVSNILRDGTTTYAGSVKGRFTISRDDAKNTVYLQMVNLKSEDTARYYCAADSGTQLGYVGAVGLSCLDYVMDYWGKGTQVTVSSHHHHHH |
Antigen
Chain: A
Mutation: S2G/Q80R/F99S/M153T/V163A
| >3G9A_A|Chain A|Green fluorescent protein|Aequorea victoria (6100) MGKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition B: GLU44 ASP95 SER96 GLY97 THR98 GLN99 ASP101 A: LYS101 HIS148 ASN149 TYR151 ILE152 THR153 LYS162 ALA163 ASN164 LYS166 ARG168 LEU178 ASP180 TYR182 ASN198 HIS199 TYR200 ALA227 GLY228 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
