Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 660 |
| PDBID: | 3TT3 |
| Chains: | HL_A |
| Organism: | Aquifex aeolicus, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 3.22 |
| Reference: | 10.1038/nature10737 |
| Antibody | |
| Antibody: | 6A10 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Leucine transporter LeuT |
| Antigen mutation: | Yes |
| Durg Target: | O67854 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >3TT3_H|Chain C[auth H]|mouse monoclonal 1gG1 Fab fragment, kappa light chain|Mus musculus (10090) QVQLQQSGTELMRPGASVKISCKAFGYTFTNHHINWMKQRPGQGLDWIGYIIPYNDYTTNNRKFKGKATLTVDRSSSTAYMELSSLTSEDSAVYYCARGNYGSAWFAYWGQGTLVTVSAAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDC |
Light Chain: L
Mutation: NULL
| >3TT3_L|Chain B[auth L]|mouse monoclonal 1gG1 Fab fragment, heavy chain|Mus musculus (10090) DIVLTQSPTYLAVSLGQRATISCRASESVDTYDNSFIHWYQQKPGQPPKLLIYRASNLESGIPARFSGSGSRTDFTLTINPVETDDVATYYCQQSNEDPLTFGSGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: A
Mutation: Y268A/K288A/T354V/S355A
| >3TT3_A|Chain A|Leucine transporter LeuT|Aquifex aeolicus (63363) MEVKREHWATRLGLILAMAGNAVGLGNFLRFPVQAAENGGGAFMIPYIIAFLLVGIPLMWIEWAMGRYGGAQGHGTTPAIFYLLWRNRFAKILGVFGLWIPLVVAIYYVYIESWTLGFAIKFLVGLVPEPPPNATDPDSILRPFKEFLYSYIGVPKGDEPILKPSLFAYIVFLITMFINVSILIRGISKGIERFAKIAMPTLFILAVFLVIRVFLLETPNGTAADGLNFLWTPDFEKLKDPGVWIAAVGQIFFTLSLGFGAIITYASAVRKDQDIVLSGLTAATLNEAAEVILGGSISIPAAVAFFGVANAVAIAKAGAFNLGFITLPAIFSQTAGGTFLGFLWFFLLFFAGLVASIAIMQPMIAFLEDELKLSRKHAVLWTAAIVFFSAHLVMFLNKSLDEMDFWAGTIGVVFFGLTELIIFFWIFGADKAWEEINRGGIIKVPRIYYYVMRYITPAFLAVLLVVWAREYIPKIMEETHWTVWITRFYIIGLFLFLTFLVFLAERRRNHESAGTLVPR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: TYR32 ASP33 ASN34 PHE36 ARG54 SER60 GLY61 H: GLN1 GLY26 TYR27 THR28 PHE29 THR30 ASN31 HIS32 HIS33 TYR50 ILE52 TYR54 TYR57 ARG74 SER75 SER77 ASN100 TYR101 GLY102 ALA104 A: TYR68 GLY70 ALA71 GLN72 GLY73 HIS74 GLY75 LEU83 ARG86 GLY186 ILE187 SER188 GLU192 THR264 TYR265 SER267 ALA268 VAL269 ARG270 GLN361 ILE364 ALA365 GLU368 ASP369 LYS372 LEU373 SER374 ARG375 PHE427 LYS431 GLU434 GLU435 ARG438 ARG508 GLU511 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
