Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6591 |
| PDBID: | 2LTQ |
| Chains: | FE_D |
| Organism: | Escherichia coli K-12, Mus musculus |
| Method: | NMR |
| Resolution (Å): | |
| Reference: | 10.1016/j.jmb.2013.02.009 |
| Antibody | |
| Antibody: | 2LTQ Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Disulfide bond formation protein B (DsbB) |
| Antigen mutation: | Yes |
| Durg Target: | P0A6M2 |
Sequence information
Antibody
Heavy Chain: F
Mutation: NULL
| >2LTQ_F|Chain C, F|Fab fragment heavy chain|Mus musculus (10090) EVQLVESGGGLVKPGGSLKLSCAASGFAFSSYDMSWVRQTPEKRLEWVAYISSGGGSTYYPDTVKGRFTISRDNAKNTLYLQMSSLKSEDTAMYYCARPDYRSYAMDYWGQGTSVTVSSAKTTAPSVYPLAPVCGDTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEPRGP |
Light Chain: E
Mutation: NULL
| >2LTQ_E|Chain B, E|Fab fragment light chain|Mus musculus (10090) MDSQAQVLILLLLWVSGTCGDIVMSQSPSSLAVSAGEKVTMSCKSSQSLLNSRTRKNYLAWYQQKPGQSPKLLIYWASTRESGVPDRFTGSGSGTDFTLTISSVQAEDLAVYYCKQSYNLYTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: D
Mutation: C8A/C41S/C49V
| >2LTQ_D|Chain A, D|Disulfide bond formation protein B|Escherichia coli (strain K12) (83333) MLRFLNQASQGRGAWLLMAFTALALELTALWFQHVMLLKPSVLCIYERVALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFATCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLVVISQPFKAKKRDLFGR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| E: ASN51 SER52 ARG53 THR54 LYS56 TYR58 TRP76 SER117 TYR118 ASN119 LEU120 TYR121 F: TYR50 TYR59 TYR101 ARG102 TYR104 D: MET93 LEU94 GLN95 LEU96 TYR97 PRO98 SER99 PRO100 PHE101 ALA102 THR103 GLN134 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
