Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6574 |
| PDBID: | 1W72 |
| Chains: | IM_D |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.15 |
| Reference: | 10.1074/jbc.M411323200 |
| Antibody | |
| Antibody: | Hyb3 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | HLA class I histocompatibility antigen |
| Antigen mutation: | No |
| Durg Target: | P04439 |
Sequence information
Antibody
Heavy Chain: I
Mutation: NULL
| >1W72_I|Chain G[auth H], H[auth I]|HYB3 HEAVY CHAIN|HOMO SAPIENS (9606) EVQLVESGGGLVQPGRSLRLSCAASGFTFDDYAMHWVRQAPGKGLEWVSGISWNSGSIGYADSVKGRFTISRDNAKNSLYLQMNSLRAEDTAVYYCARGRGFHYYYYGMDIWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPK |
Light Chain: M
Mutation: NULL
| >1W72_M|Chain I[auth L], J[auth M]|HYB3 LIGHT CHAIN|HOMO SAPIENS (9606) SYVLTQPPSVSVAPGQTARITCGGNNIGSRSVHWYQQKPGQAPVLVVYDDSDRPSGIPERFSGSNSGNMATLTISRVEAGDEADYYCQVWDSRTDHWVFGGGTDLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADGSPVKAGVETTKPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAP |
Antigen
Chain: D
Mutation: NULL
| >1W72_D|Chain A, D|HLA CLASS I HISTOCOMPATIBILITY ANTIGEN|HOMO SAPIENS (9606) GSHSMRYFFTSVSRPGRGEPRFIAVGYVDDTQFVRFDSDAASQKMEPRAPWIEQEGPEYWDQETRNMKAHSQTDRANLGTLRGYYNQSEDGSHTIQIMYGCDVGPDGRFLRGYRQDAYDGKDYIALNEDLRSWTAADMAAQITKRKWEAVHAAEQRRVYLEGRCVDGLRRYLENGKETLQRTDPPKTHMTHHPISDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDGTFQKWAAVVVPSGEEQRYTCHVQHEGLPKPLTLRW |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| I: ASP30 ASP31 TRP52 SER54 GLY55 ILE57 ARG96 GLY97 PHE98 HIS99 M: ASN27 GLY29 SER30 ASP50 TRP91 SER93 ARG94 D: GLN62 ARG65 ASN66 LYS68 ALA69 GLN72 THR73 ARG75 ALA76 ASN77 GLY79 THR80 ARG82 GLY83 TYR84 ASN86 GLN87 MET138 ILE142 LYS146 VAL150 GLN155 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
