Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6564 |
| PDBID: | 7SLA |
| Chains: | B_A |
| Organism: | Homo sapiens, synthetic construct |
| Method: | EM |
| Resolution (Å): | 3.15 |
| Reference: | 10.1038/s41586-021-04211-w |
| Antibody | |
| Antibody: | Nb1 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Sodium/glucose cotransporter 1 (SGLT1 ) |
| Antigen mutation: | No |
| Durg Target: | P13866 |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >7SLA_B|Chain B|nanobody Nb1|synthetic construct (32630) MAQVQLQESGGGLVQAGGSLRLSCAASGTIFVFDKMGWYRQAPGKEREFVATISRGGSTNYADSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCAVRYTPWRRYSYWGQGTQVTVSSHHHHHH |
Antigen
Chain: A
Mutation: NULL
| >7SLA_A|Chain A|Sodium/glucose cotransporter 1|Homo sapiens (9606) MDSSTWSPKTTAVTRPVETHELIRNAADISVIVIYFLLVMAVGLWSMFKTNRGTVGGFFLAGRSMVWWPIGASLFASNIGSGHFIGLAGTGAASGLAVGGFEWNALVLLLVLGWVFVPIYIKAGVVTMPEYLRKRFGGQRIQVYLSVLSLFLYIFTKISVDIFSGAIFINLALGWNLYLSIILLLAITALYTITGGLAAVIYTDTLQTLIMLIGALILMGFAFHEVGGYDAFMEKYMKAIPTIVSDGNTTFQEKCYTPRADSFHIFRDPLTGDLPWPGFIFGLTILALWYWCTDQVIVQRCLAAKNMSHVKGGCILAGYLKLLPMFIMVMPGMISRILFPDKVACVVPSECEKYCGTKVGCTNIAYPTLVVELMPNGLRGLMLAVMLAALMSSLTSIFNSASTLFTMDIYAKVRKRASEKELMIVGRLFVLFLVVVSIAWIPIVQSAQSGQLFDYIQSVSSYLAPPVAAVFLLAIFWKRVNEQGAFWGLILGLLLGLSRLILEFAYGTGSCMEPSNCPTIICGVHYLYFAIILFAISGIVTVVVSLLTKPIPDVHLYRLCWSLRNSKEERIDLDAEEENIQEGPKETIEIETQVPEKKKGIFRRAYDLFCGLEQHGAPKMTEEEEKAMKMKMTDTSEKPLWRTVLNINAILLLAVAIFCHAYFASNSLEVLFQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: VAL4 GLY28 THR29 ILE30 PHE31 VAL32 ARG55 ALA76 LYS77 ASN78 TYR101 THR102 PRO103 TRP104 ARG105 SER108 TYR109 A: TYR229 ASP230 MET233 GLU234 MET237 LYS238 PHE263 PHE658 ALA661 TYR662 SER665 ASN666 SER667 LEU668 GLU669 VAL670 LEU671 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
