Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6563 |
| PDBID: | 7SL9 |
| Chains: | B_A |
| Organism: | Homo sapiens, synthetic construct |
| Method: | EM |
| Resolution (Å): | 3.50 |
| Reference: | 10.1038/s41586-021-04211-w |
| Antibody | |
| Antibody: | Nb2 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Sodium-coupled monocarboxylate transporter 1 (SMCT1) |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >7SL9_B|Chain B|nanobody Nb2|synthetic construct (32630) MAQVQLQESGGGLVQAGGSLRLSCAASGNISTRAGMGWYRQAPGKEREFVASINWGAITNYADSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCAVEYKYGPQRSDTYYYWGQGTQVTVSSHHHHHH |
Antigen
Chain: A
Mutation: NULL
| >7SL9_A|Chain A|Sodium-coupled monocarboxylate transporter 1|Homo sapiens (9606) MDTPRGIGTFVVWDYVVFAGMLVISAAIGIYYAFAGGGQQTSKDFLMGGRRMTAVPVALSLTASFMSAVTVLGTPSEVYRFGAIFSIFAFTYFFVVVISAEVFLPVFYKLGITSTYEYLELRFNKCVRLCGTVLFIVQTILYTGIVIYAPALALNQVTGFDLWGAVVATGVVCTFYCTLGGLKAVIWTDVFQVGIMVAGFASVIIQAVVMQGGISTILNDAYDGGRLNFWNFNPNPLQRHTFWTIIIGGTFTWTSIYGVNQSQVQRYISCKSRFQAKLSLYINLVGLWAILTCSVFCGLALYSRYHDCDPWTAKKVSAPDQLMPYLVLDILQDYPGLPGLFVACAYSGTLSTVSSSINALAAVTVEDLIKPYFRSLSERSLSWISQGMSVVYGALCIGMAALASLMGALLQAALSVFGMVGGPLMGLFALGILVPFANSIGALVGLMAGFAISLWVGIGAQIYPPLPERTLPLHLDIQGCNSTYNETNLMTTTEMPFTTSVFQIYNVQRTPLMDNWYSLSYLYFSTVGTLVTLLVGILVSLSTGGRKQNLDPRYILTKEDFLSNFDIFKKKKHVLSYKSHPVEDGGTDNPAFNHIELNSDQSGKSNGTRLSNSLEVLFQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: GLN3 ASN29 ILE30 SER31 THR32 ARG33 ALA34 ASN54 TRP55 GLY56 TYR101 LYS102 TYR103 GLY104 PRO105 THR110 TYR111 TYR113 A: ARG80 ASP220 TYR222 ASP223 GLY224 GLY225 LEU227 ASN228 TRP230 ASN231 TYR302 HIS306 ASP307 TRP311 THR312 LYS315 PRO467 GLU468 LEU471 PRO472 LEU473 HIS474 LEU475 ASP476 ILE477 GLN478 GLY479 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
