Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 655 |
| PDBID: | 3T2N |
| Chains: | IM_B |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.55 |
| Reference: | 10.1042/BJ20111317 |
| Antibody | |
| Antibody: | hH35 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Serine protease hepsin |
| Antigen mutation: | No |
| Durg Target: | P05981 |
Sequence information
Antibody
Heavy Chain: I
Mutation: NULL
| >3T2N_I|Chain C[auth H], D[auth I]|Antibody, Fab fragment, Heavy Chain|Homo sapiens (9606) QVQLVQSGSELKKPGASVKVSCKASGYTFTDYSMRWVRQAPGQGLEWMGWINTETGSPTYADDFKGRFVFSLDTSVSTAYLQISSLKAEDTAVYYCARGFAYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPC |
Light Chain: M
Mutation: NULL
| >3T2N_M|Chain E[auth L], F[auth M]|Antibody, Fab fragment, Light Chain|Homo sapiens (9606) QTVVTQEPSLTVSPGGTVTLTCRSSTGAVTTSNYANWVQQKPGQAFRGLIGDTNNRAPWTPARFSGSLLGGKAALTLSGVQPEDEAEYYCALWYSNHFIFGSGTKVTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: B
Mutation: NULL
| >3T2N_B|Chain A, B|Serine protease hepsin|Homo sapiens (9606) SDQEPLYPVQVSSADARLMVFDKTEGTWRLLCSSRSNARVAGLSCEEMGFLRALTHSELDVRTAGANGTSGFFCVDEGRLPHTQRLLEVISVCDCPRGRFLAAICQDCGRRKLPVDRIVGGRDTSLGRWPWQVSLRYDGAHLCGGSLLSGDWVLTAAHCFPERNRVLSRWRVFAGAVAQASPHGLQLGVQAVVYHGGYLPFRDPNSEENSNDIALVHLSSPLPLTEYIQPVCLPAAGQALVDGKICTVTGWGNTQYYGQQAGVLQEARVPIISNDVCNGADFYGNQIKPKMFCAGYPEGGIDACQGDSGGPFVCEDSISRTPRWRLCGIVSWGTGCALAQKPGVYTKVSDFREWIFQAIKTHSEASGMVTQL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| M: THR29 TYR32 ASN34 GLY46 ILE48 GLY49 ASP50 THR51 ASN52 ASN53 ARG54 ALA55 PRO56 LEU66 TRP91 SER93 ASN94 PHE96 I: THR30 ASP31 TYR32 SER33 ARG35 TRP47 TRP50 THR52 GLU53 THR58 ARG94 GLY95 PHE96 ALA101 TYR102 B: GLU252 ILE317 ASP320 VAL321 ASN323 GLY324 ALA325 ASP326 PHE327 TYR328 GLY329 ASN330 GLN331 LYS333 PRO334 TYR341 ALA384 GLN385 LYS386 PRO387 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
