Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6542 |
| PDBID: | 7SAK |
| Chains: | B_A |
| Organism: | Discosoma sp., Lama glama |
| Method: | XRD |
| Resolution (Å): | 1.15 |
| Reference: | 10.1002/pro.4383 |
| Antibody | |
| Antibody: | LaM4 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | mCherry |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >7SAK_B|Chain B|LaM4|Lama glama (9844) MGSSHHHHHHSSGLVPRGSMAQVQLVESGGSLVQPGGSLRLSCAASGRFAESSSMGWFRQAPGKEREFVAAISWSGGATNYADSAKGRFTLSRDNTKNTVYLQMNSLKPDDTAVYYCAANLGNYISSNQRLYGYWGQGTQVTVS |
Antigen
Chain: A
Mutation: NULL
| >7SAK_A|Chain A|mCherry|Discosoma sp. (86600) SNGMVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition B: ARG29 PHE30 GLU32 SER33 TRP55 SER56 ALA59 THR60 ASN61 LEU102 GLY103 ASN104 TYR105 ILE106 SER107 ASN109 LEU112 GLY114 TYR115 A: ASN4 ALA6 ILE7 ILE8 LYS9 GLU10 TYR38 ALA77 ASP78 ILE79 PRO80 ASP81 LYS84 LEU85 PHE87 PRO88 LYS185 PRO186 VAL187 GLN188 LEU189 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
