Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6536 |
| PDBID: | 7SAI |
| Chains: | C_A |
| Organism: | Aequorea victoria, Lama glama |
| Method: | XRD |
| Resolution (Å): | 2.23 |
| Reference: | 10.1002/pro.4383 |
| Antibody | |
| Antibody: | Lag30 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Green fluorescent protein |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Chain: C
Mutation: NULL
| >7SAI_C|Chain B[auth C]|LAG30 Nanobody|Lama glama (9844) MGSSHHHHHHSSGLVPRGSMAQVQLVESGGGLVQAGGSLRLSCAASGRTFSTSAMGWFRQAPGREREFVAAITWTVGNTIYGDSMKGRFTISRDRTKNTVDLQMDSLKPEDTAVYYCTARSRGFVLSDLRSVDSFDYKGQGTQVTVS |
Antigen
Chain: A
Mutation: F65L/S66T
| >7SAI_A|Chain A|Green fluorescent protein|Aequorea victoria (6100) GVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition C: ARG29 THR30 PHE31 SER32 THR33 SER34 THR54 TRP55 THR56 VAL57 ASN59 ARG76 ARG101 SER102 ARG103 GLY104 PHE105 VAL106 LEU107 ASP109 A: LEU7 LEU15 GLU17 PRO89 GLU90 ARG109 ALA110 GLU111 LYS113 PHE114 GLU115 GLY116 ASP117 THR118 VAL120 ARG122 ILE123 GLU124 ILE188 GLY189 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
