Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6533 |
| PDBID: | 7S8H |
| Chains: | HL_a |
| Organism: | Lassa virus Josiah, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.70 |
| Reference: | 10.1016/j.celrep.2022.110841 |
| Antibody | |
| Antibody: | 18.5C Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Lassa virus glycoprotein G1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7S8H_H|Chain B[auth H]|18.5C Fab Heavy Chain|Homo sapiens (9606) DEVQLVQSGGGLVRPGGSLRLSCAAAGFTFKSYSMNWVRQAPGRGLEWVSSITSGGSKTYYADVVKGRFTVSRDNAKQSLYLQMNSLRAEDTAIYFCARSLHSTSQPSYMDVWGRKITVIVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDK |
Light Chain: L
Mutation: NULL
| >7S8H_L|Chain C[auth L]|18.5C Fab Light Chain|Homo sapiens (9606) DDIVLTQSPGTLSLSPGERATLSCRASQSVISYYVAWYQHKGGQAPRLLIYGASSRATGVPDRFSGSGSGTDFTLTISSLEPEDFALYYCQYYGSSPLWAFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: a
Mutation: NULL
| >7S8H_a|Chain F[auth a]|Glycoprotein G2|Lassa virus (11622) GTFTWTLSDSEGKDTPGGYCLTRWMLIEAELKCFGNTAVAKCNEKHDEEFCDMLRLFDFNKQAIQRLKAPAQTSIQLINKAVNALINDQLCMKNHLRDIMGIPYCNYSKYWYLNHTTTGRTSLPKCWLVSNGSYLNETHFSDDIEQQADNMITEMLQKEYMERQG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition H: SER34 THR53 SER54 GLY55 SER57 LYS58 TYR60 LEU101 HIS102 SER103 THR104 SER105 L: SER28 ILE30 SER31 TYR32 TYR33 GLY69 TYR91 TYR92 GLY93 SER94 LEU97 a: ARG356 GLY360 ILE361 PRO362 LEU387 SER389 ASN390 SER392 TYR393 LEU394 ASN395 GLU396 THR397 HIS398 PHE399 SER400 ASP401 ASP402 GLU404 ASP408 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
