Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6531 |
| PDBID: | 7S6D |
| Chains: | EG_A |
| Organism: | Saccharopolyspora erythraea, Streptomyces lasalocidi, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.10 |
| Reference: | 10.1126/science.abi8532 |
| Antibody | |
| Antibody: | 1B2 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | 6-deoxyerythronolide-B synthase EryA2, modules 3 and 4, Lsd14 Polyketide synthase fusion |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: E
Mutation: NULL
| >7S6D_E|Chain D, F[auth E]|Fab 1B2 heavy chain|Homo sapiens (9606) MAEVQLVQSGGGLVQPGRSLRLSCTASGFTFGDYAMSWVRQAPGKGLEWVGFIRSKAYGGTTEYAASVKGRFTISRDDSKSIAYLQMNSLKTEDTAVYYCTRGGTLFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCAALVPRGSAHHHHHHAADYKDDDDKA |
Light Chain: G
Mutation: NULL
| >7S6D_G|Chain E[auth F], G|Fab 1B2 light chain|Homo sapiens (9606) LFAIPLVVPFYSHSALDVVMTQSPLSLPVTPGEPASISCRSSQSLLHSNGYNYLDWYLQKPGQSPQLLIYLGSNRASGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCMQSLQTPRLTFGPGTKVDIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPRGAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >7S6D_A|Chain A, B, C|6-deoxyerythronolide-B synthase EryA2, modules 3 and 4, Lsd14 Polyketide synthase fusion|Saccharopolyspora erythraea (1836) MVTDSEKVAEYLRRATLDLRAARQRIRELESEPIAIIGMACRLPGGVDSPEGLWELVDSGTDAIAGFPLDRGWDVEGMYDPDAEAPGKTYVKEAGFLYDAGEFDAGFFGISPREAVSMDPQQRLMLEASWEAFERAGLDPARQRGTATGVFVGATATGYVSPAAEVPEGAEGFAITGNMTAVTSGRISYTLGLQGPAVTIDTACSSSLVALHLACQSLRQGECTTALAGGVTVMPTPTAFTEFSRQRGLAPDGRCKSFAAAADGTNWAEGVAVLVVERLSDARRNGHRVLAVVRGTAINQDGASNGLSAPNDLAQERVIRSALDNAGLTASDVDAVEAHGTGTTLGDPIEAQALLAAYGHERPAHRPLRVGSLKSNIGHAGPAAGVAGVIKMVMAMRHGVLPRSLHIDEPTPQVDWSSGAVTLLTEPVDWPDSDRPRRAGVSAFGISGTNAHVILEQAPTQDPQQPAPPVPAAPWLLSAKTPAALRAQARRLHTHLARHPHPDPTDIAHALATTRTPHEHRAALVTDDHGTRGPALAALAEGAPDACLISGTALSKGRTVFVFPGQGSQWTGMGRELLHTSPEFAAYIAECETALNDFVDWSLTDVLRGTEGAPGYDRVDVVQPALFAVMVSLARLWQHHGIHPDAVIGHSQGEIAAAHIAGALSLQDAARIVALRSQALLPLAGLGGMTSLALPHDQALQLIQPWGQDLSIASVNGPHSTVVSGTTHALDELHTTCDTQGVRARRIPVDYASHSAQVESIRDTVLQAATGINPQPTTIPLYSTVTGQPIDGTQLDADYWYTNLRHTVRFEETTRALLGSGHRHFIETTAHPVLALALEETIEATGSDARVTGTLRRDHGDLTQLHTALATAWTHGIDVDWTAVLGDRRTPFELPTYAFQRQRYWLEPGAGVGVPVGGTSAEARFWDAVEDEDLEALVAAIGADGDDASWAGVLPALAGWRRRQREQSALDDLRYKVTWKPTAVADGASATGTWLVVVPESLAGGGWPVVVARAVDQAGGRPVVLSVDAADGADRSRLGLRIHEALGEGPVPDAVVSLLALDPSALPGLPDVPQALASTAALVQALLDLGLEARLWCVTSGAVSVSGADGPSAPEQAAVWGFGRVAGLEHPHLWAGLVDLPPEADERTAARLVGVLAGAGGEDQVALRSSGVFVRRLVRAPASEVPAVRSWKPGGTVLVTGGTGGLGRQVARWLARGGADHLLLVSRRGVDAPGADELVDELTDLGARVTVAACDVADRDAVQRLLSEQVPSDAPLTAVIHTAAVLDDGVIDSLSPERMEQVLRVKVGGAVHLYELTRESDLSAFVLFSSFGSTFGLPGLGNYAPGNAALEALAEQWRAEGRPATAVGWGTWAGGGMADGGVGERGRTHGIHELEPALATAALEQALERDESSPVIIDIDWERFAVAFHAKRPTRGFELVPEAQAALEAADGGPGPDGGAGDPSELLDRLAALPDAERDRALLEVVRGNAASVMSHGAMRTATLEAVEPTRAFRDLGFDSLMAVELRNRIGAATGLRLAPTLVFDHPTPEAVVRHLRAELGLEGDGAPDPVFDELDGLERALSSYTPDTDTRVKITKRLESLLWEWTRSEADAPDPVDAADLAAVSDDEMFELIDRELGSALEHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition E: VAL4 ASP33 TYR34 ALA35 PHE52 ARG54 TYR58 ARG102 GLY103 GLY104 THR105 LEU106 ASP108 ASN162 SER163 LYS213 G: HIS47 ASN49 TYR51 TYR53 ASP55 LEU67 TYR70 LEU71 ARG75 ALA76 SER77 SER112 LEU113 THR115 A: VAL8 THR9 ASP10 SER11 GLU12 LYS13 VAL14 ALA15 GLU16 TYR17 ARG19 ARG20 ALA21 LEU23 ASP24 ALA27 ASN779 GLN781 PRO782 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
