Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 653 |
| PDBID: | 3STB |
| Chains: | B_C |
| Organism: | Lama glama, Trypanosoma brucei |
| Method: | XRD |
| Resolution (Å): | 2.50 |
| Reference: | 10.1093/nar/gkr867 |
| Antibody | |
| Antibody: | Nb14 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | RNA-editing complex protein MP42 (A3) |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >3STB_B|Chain A, B|single domain antibody VHH|Lama glama (9844) QVQLQESGGGLVQAGGSLRLSCAASGRTLSSYAMGWFRQAPGKEREFVAAINRSGSTFYADAVKGRFTISRDNAKNTVYLQMNSLKPEDTAAYYCAADRFSPVVPGPIPVNTVDSWGQGTQVTVSSHHHHHH |
Antigen
Chain: C
Mutation: NULL
| >3STB_C|Chain C|RNA-editing complex protein MP42|Trypanosoma brucei (5691) MRSAMGTQYVHGQETILPQAPQYHLDVAPNAPEEGEVAAHWRCVNHCVMLGVVQNIQEGFVFEDKVLQFTLITDFEGPSPGDPDKDFHTVRVFDSDYSSRVKEQLRDGEWFLVTGRLRMVPQYDGSMRKYYHYPVIQVHPGCGSVLKV |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: GLN3 VAL4 GLN5 LEU6 ARG29 SER33 TYR34 ARG47 ARG55 SER56 TYR96 ASP100 ARG101 PHE102 SER103 PRO104 VAL105 VAL106 GLY108 PRO111 ASN113 THR114 VAL115 ASP116 SER117 TRP118 GLY119 GLN120 C: ASN300 GLN302 GLU303 GLY304 PHE305 VAL306 PHE307 GLU308 VAL311 GLN313 THR334 ARG336 VAL365 PRO366 GLN367 TYR368 ASP369 GLY370 SER371 ARG373 TYR375 TYR378 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
