Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6528 |
| PDBID: | 7S2S |
| Chains: | A_D |
| Organism: | Camelus bactrianus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 1.93 |
| Reference: | 10.1016/j.cell.2022.02.025 |
| Antibody | |
| Antibody: | IL2Rb-binding Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Interleukin-2 receptor subunit beta |
| Antigen mutation: | No |
| Durg Target: | P14784 |
Sequence information
Antibody
Chain: A
Mutation: NULL
| >7S2S_A|Chain A, C|IL2Rb-binding nanobody|Camelus bactrianus (9837) QVQLQESGGGSVQAGGSLRLSCAASSYTISSVCMGWFRQAPGKEREGVAGIAPDGSTGYGDSVKGRFTISKDNAKNTLYLQMNSLKPEDTAMYYCAAASPGRCFLPRTALEPALYYNWGQGTQVTVSSGAPGSGLNDIFEAQKIEWHEHHHHHH |
Antigen
Chain: D
Mutation: NULL
| >7S2S_D|Chain B, D|Interleukin-2 receptor subunit beta|Homo sapiens (9606) AVNGTSQFTCFYNSRANISCVWSQDGALQDTSCQVHAWPDRRRWNQTCELLPVSQASWACNLILGAPDSQKLTTVDIVTLRVLCREGVRWRVMAIQDFKPFENLRLMAPISLQVVHVETHRCNISWEISQASHYFERHLEFEARTLSPGHTWEEAPLLTLKQKQEWICLETLTPDTQYEFQVRVKPLQGEFTTWSPWSQPLAFRTKPAALHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition A: GLU44 PRO100 GLY101 ARG102 CYS103 PHE104 LEU105 ARG107 THR108 GLU111 ALA113 LEU114 TYR116 D: SER32 GLN33 PHE34 THR35 TRP64 ASP66 ILE103 THR105 ARG107 ARG115 ARG117 VAL118 MET119 ALA120 ILE121 GLN122 ASP123 LYS125 GLU216 PHE217 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
