Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6520 |
| PDBID: | 7RP2 |
| Chains: | HI_A |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.20 |
| Reference: | 10.1038/s41587-021-01126-9 |
| Antibody | |
| Antibody: | 2H11 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | GTPase Kras |
| Antigen mutation: | Yes |
| Durg Target: | P01116; P01112; |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7RP2_H|Chain B[auth H]|immunoglobulin IgG heavy chain|Homo sapiens (9606) EVQLQESGPGLVKPPGTLSLTCAVSGGSISSSNWWSWVRQPPGKGLEWIGEIYHSGSTNYNPSLKSRVTISVDKSKNQFSLKLSSVTAADTAVYYCARGSSSWYDLGPFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCD |
Light Chain: I
Mutation: NULL
| >7RP2_I|Chain C[auth I]|immunoglobulin IgG light chain|Homo sapiens (9606) SVLTQPPSASGTPGQRVTISCSGSSSNIGSNYVYWYQQLPGTAPKLLIYRNNQRPSGVPDRFSGSKSGTSASLAISGLRSEDEADYYCAAWDERLSGWVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: A
Mutation: G13C/C52S/C81L/C119S
| >7RP2_A|Chain A|GTPase KRas|Homo sapiens (9606) GMTEYKLVVVGACGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETSLLDILDTAGQEEYSAMRDQYMRTGEGFLLVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKSDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: GLU1 GLY26 GLY27 SER28 SER30 SER31 SER32 ASN33 TYR52 HIS53 ARG94 SER97 SER98 TRP99 I: TYR49 GLN53 ARG54 PRO55 SER56 A: LYS5 LEU6 VAL7 GLN25 TYR32 ASP33 THR35 ILE36 GLU37 ASP38 SER39 TYR40 ARG41 ASP54 ILE55 LEU56 SER65 MET67 GLN70 TYR71 THR74 GLY75 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
