Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 652 |
| PDBID: | 3STB |
| Chains: | A_D |
| Organism: | Lama glama, Trypanosoma brucei |
| Method: | XRD |
| Resolution (Å): | 2.50 |
| Reference: | 10.1093/nar/gkr867 |
| Antibody | |
| Antibody: | Nb14 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | MP18 RNA editing complex protein (A6) |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: A
Mutation: NULL
| >3STB_A|Chain A, B|single domain antibody VHH|Lama glama (9844) QVQLQESGGGLVQAGGSLRLSCAASGRTLSSYAMGWFRQAPGKEREFVAAINRSGSTFYADAVKGRFTISRDNAKNTVYLQMNSLKPEDTAAYYCAADRFSPVVPGPIPVNTVDSWGQGTQVTVSSHHHHHH |
Antigen
Chain: D
Mutation: NULL
| >3STB_D|Chain D|MP18 RNA editing complex protein|Trypanosoma brucei (5691) KSVNSVTLVGVVHDIQSGFVYEDAVTQFTLTTTSIDTTHPTQEVVVEKDHHTIRCFGELFSAEVKQKVKEGNVVCVNGRLRLSPQLEPSCNKHFYFPYIQVQPPHGQVAVIHGDRRTVPAAVNPAVEDIKSEKEGSGGDQSGVPS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition A: VAL4 GLN5 ARG29 SER33 TYR34 ARG55 ARG101 PHE102 SER103 PRO104 VAL105 VAL106 VAL112 ASN113 THR114 VAL115 ASP116 SER117 TRP118 GLY119 GLN120 D: GLN35 SER36 GLY37 PHE38 VAL39 TYR40 GLU41 VAL44 GLN46 THR71 ARG73 SER102 GLN104 LEU105 GLU106 SER108 CYS109 ASN110 LYS111 TYR114 PHE115 TYR117 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
