Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6516 |
| PDBID: | 7RFP |
| Chains: | IM_B |
| Organism: | Mus musculus, Muromegalovirus G4 |
| Method: | EM |
| Resolution (Å): | 4.40 |
| Reference: | 10.1126/sciadv.abm4552 |
| Antibody | |
| Antibody: | DTA-1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Tumor necrosis factor receptor superfamily member 18 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: I
Mutation: NULL
| >7RFP_I|Chain C[auth H], E[auth I]|DTA-1 (heavy chain)|Mus musculus (10090) MGWSCIILFLVATATGVHSELQLVESGGGLVQPGRSLKLSCSASGFIFSNSYMAWVRQAPKKGLEWVATINPSGSRTYYPDSVKGRFTISRDTAKSSLYLQMNSLKSEDTATYYCARHDLSFDYWGQGVMVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK |
Light Chain: M
Mutation: NULL
| >7RFP_M|Chain D[auth L], F[auth M]|DTA-1 (light chain)|Mus musculus (10090) MGWSCIILFLVATATGVHSQFTLTQPKSVSGSLRSTITIPCDRSSGGIRDSYVSWYQQHLGRPPLNVIYADDQRPSEVSDRFSGSIDSSSNSASLTITNLQMDDEADYFCQSYDSDFDVYIFGGGTKLTVLGQRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: B
Mutation: NULL
| >7RFP_B|Chain A, B|Tumor necrosis factor receptor superfamily member 18,Enhanced green fluorescent protein|Mus musculus (10090) MGAWAMLYGVSMLCVLDLGQPSVVEEPGCGPGKVQNGSGNNTRCCSLYAPGKEDCPKERCICVTPEYHCGDPQCKICKHYPCQPGQRVESQGDIVFGFRCVACAMGTFSAGRDGHCRLWTNCSQFGFLTMFPGNKTHNAVCIPEPLPTEQYGHLTVIFLVMAACIFFLTTVQLGLHIWQLRRQHMCPRETQPFAEVQLSAEDACSFQFPEEERGEQTEEKCHLGGRWPAGLVPRGSAAAMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRHHHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| I: TYR52 SER73 HIS118 ASP119 LEU120 M: ILE48 ASP50 SER51 TYR52 ALA70 SER112 TYR113 ASP114 SER115 ASP116 PHE117 B: ASP54 ASP71 ILE76 LYS78 HIS79 TYR80 GLN91 GLY92 ASP93 ILE94 VAL95 PHE96 GLY97 PHE98 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
