Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6493 |
| PDBID: | 8X0T |
| Chains: | HL_A |
| Organism: | Homo sapiens, synthetic construct |
| Method: | XRD |
| Resolution (Å): | 2.50 |
| Reference: | 10.1016/j.ijbiomac.2023.127846 |
| Antibody | |
| Antibody: | pF8_AC3 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human cysteine-rich domain (CRD) of frizzled receptor 8 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >8X0T_H|Chain B[auth H]|pF8_AC3 Heavy chain|synthetic construct (32630) EVQLVESGGGLVQPGGSLRLSCAASGFTFSSFNMFWVRQAPGKGLEWVAGIDDDGSYPNYGSAVKGRATISRDNSKNTLYLQMNSLRAEDTAVYYCAKSGPRPPHTTYWHKSIDAWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCHHHHHHHH |
Light Chain: L
Mutation: NULL
| >8X0T_L|Chain C[auth L]|pF8_AC3 Light chain|synthetic construct (32630) ELTQPPSVSVSPGQTARITCSGDGSWCPFCFYGWYQQKPGQAPVTLIYLNNKRPSGIPERFSGSLSGSTNTLTISGVQAEDEADYYCGSSDFRRWAFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: A
Mutation: NULL
| >8X0T_A|Chain A|Frizzled-8|Homo sapiens (9606) AKELACQEITVPLCKGIGYQYTYMPNQFNHDTQDEAGLEVHQFWPLVEIQCSPDLKFFLCSMYTPICLEDYKKPLPPCRSVCERAKAGCAPLMRQYGFAWPDRMRCDRLPEQGNPDTLCMDYNRHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: PRO101 ARG102 PRO103 PRO104 HIS105 THR106 THR107 TYR108 TRP109 HIS110 L: SER25 TRP26 CYS27 PRO28 PHE29 CYS30 PHE31 LEU49 ASN50 ASN51 LYS52 SER64 LEU65 SER66 GLY67 SER68 ASP91 PHE92 ARG93 A: HIS59 LEU67 GLU68 HIS70 GLN71 PHE72 TRP73 PRO74 LEU75 GLU77 ILE78 TYR92 LEU121 MET122 GLN124 TYR125 GLY126 PHE127 ALA128 PRO130 ASP131 ARG132 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
