Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6468 |
| PDBID: | 8TRT |
| Chains: | AG_E |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.00 |
| Reference: | 10.1002/pro.4824 |
| Antibody | |
| Antibody: | FabC1 S1CE variant Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human N-terminal cysteine-rich domain of ephrin type-A receptor 2 (EPHA2-CRD) |
| Antigen mutation: | No |
| Durg Target: | P29317 |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >8TRT_A|Chain A, C[auth B]|S1CE variant of Fab C1 heavy chain|Homo sapiens (9606) EVQLVESGGGLVQPGGSLRLSCAASGFTIYYSSMHWVRQAPGKGLEWVASISSYYGYTYYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARYYAMDYWGQGTLVTVFNQIKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHT |
Light Chain: G
Mutation: NULL
| >8TRT_G|Chain B[auth C], D[auth G]|S1CE variant of Fab C1 light chain|Homo sapiens (9606) DIQMTQSPSSLSASVGDRVTITCRASQSVSSAVAWYQQKPGKAPKLLIYSASSLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQYYGYGGYPITFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVSWYVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTQGTTSVTKSFNRGEC |
Antigen
Chain: E
Mutation: NULL
| >8TRT_E|Chain E[auth D], F[auth E]|Ephrin type-A receptor 2|Homo sapiens (9606) KKCPELLQGLAHFPETIAGSDAPSLATVAGTCVDHAVVPPGGEEPRMHCAVDGEWLVPIGQCLCQAGYEKVEDACQACSPGFFKFEASESPCLECPEHTLPSPEGATSCECEEGFFRAPQDPASMPCTLVPR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: TYR32 SER34 TYR59 TYR60 TYR62 TYR64 TYR107 TYR108 ALA109 G: SER30 SER31 ALA32 TYR55 SER56 SER66 TYR108 GLY109 TYR110 GLY112 TYR113 E: SER277 PRO278 GLY279 PHE280 PHE281 GLU287 SER288 PRO289 CYS290 LEU291 GLU292 CYS293 PRO294 GLU295 HIS296 THR297 LEU298 PRO299 GLU310 GLU311 PHE313 SER322 PRO324 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
