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Entry information

Complex
AACDB_ID: 646
PDBID: 3S35
Chains: HL_X
Organism: Mus musculus, Homo sapiens
Method: XRD
Resolution (Å): 2.20
Reference: 10.1016/j.str.2011.01.019
Antibody
Antibody: 6.64 Fab
Antibody mutation: No
INN (Clinical Trial):
Antigen
Antigen: Vascular endothelial growth factor receptor 2
Antigen mutation: No
Durg Target: P35968

Sequence information

Antibody

Heavy Chain: H
Mutation: NULL

>3S35_H|Chain A[auth H]|6.64 Fab heavy chain|Mus musculus (10090)
KVQLQQSGTELVKPGASVKVSCKASGYIFTEYIIHWVKQRSGQGLEWIGWLYPESNIIKYNEKFKDKATLTADKSSSTVYMELSRLTSEDSAVYFCTRHDGTNFDYWGQGTTLTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPR

Light Chain: L
Mutation: NULL

>3S35_L|Chain B[auth L]|6.64 Fab light chain|Mus musculus (10090)
DIVLTQSPASLAVSLGQRATISCRASESVDSYGNSFMHWYQQKPGQPPKLLIYRASNLESGIPARFSGSGSRTDFTLTINPVEADDVATYYCQQSNEDPLTFGAGTKLELKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNE

Antigen

Chain: X
Mutation: NULL

>3S35_X|Chain C[auth X]|Vascular endothelial growth factor receptor 2|Homo sapiens (9606)
ADPGYRIYDVVLSPSHGIELSVGEKLVLNCTARTELNVGIDFNWEYPSSKHQHKKLVNRDLKTQSGSEMKKFLSTLTIDGVTRSDQGLYTCAASSGLMTKKNSTFVRVHEKPFVAFGSGMES

Interaction

1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.

Interacting residues (ΔSASA based)

H: GLU31 TYR32 ILE33 TRP50 TYR52 GLU54 SER55 ILE57 ILE58 LYS59 HIS99 ASP100 GLY101 THR102 ASN103 ASP105

L: SER31 TYR32 GLY33 ASN34 PHE36 HIS38 TYR53 ARG54 ASN57 LEU58 GLU59 SER60 SER95 ASN96 ASP98

X: VAL238 GLY239 GLU240 LYS241 VAL243 ASN245 SER265 LYS266 HIS267 LYS270 LYS271 LEU272 ARG275 LYS278 THR291 THR293 ILE294 ASP295 GLY296 THR298 ARG299 SER300 GLU326 LYS327 PRO328 PHE329 VAL330

2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.

Interacting residues (Atom distance based)

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