Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6445 |
| PDBID: | 8T9Z |
| Chains: | HL_A |
| Organism: | human metapneumovirus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.00 |
| Reference: | 10.1128/jvi.01052-23 |
| Antibody | |
| Antibody: | M8C10 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human metapneumovirus fusion glycoprotein |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >8T9Z_H|Chain B[auth H]|M8C10 Fab Heavy Chain|Homo sapiens (9606) QVQLVESGGGVVQPGRSLRLSCAASGFTFTTYTMHWVRQAPGKGLEWVALISSDGNNKYYADSVKGRFTISRDNSKNTLYLQMSSLRAEDTAVYYCARGYYSGGDAFDVWGQGTMVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKHHHHHH |
Light Chain: L
Mutation: NULL
| >8T9Z_L|Chain C[auth L]|M8C10 Fab Light Chain|Homo sapiens (9606) DIQMTQSPSTLSASVGDRVTITCRASQTITIWLAWYQQKPGKAPKLLIYDAVSLESGVPSRFRGSGSGTDFTLTISSLQPDDFATYYCQQYNNYPYTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >8T9Z_A|Chain A|Fusion glycoprotein F0|Human metapneumovirus (162145) LKESYLEESCSTITEGYLSVLRTGWYTNVFTLEVGDVENLTCTDGPSLIKTELDLTKSALRELKTVSADQLAREEQIENPRQSRFVLGAIALGVATAAAVTAGIAIAKTIRLESEVNAIKGALKTTNEAVSTLGNGVRVLATAVRELKEFVSKNLTSAINKNKCDIADLKMAVSFSQFNRRFLNVVRQFSDNAGITPAISLDLMTDAELARAVSYMPTSAGQIKLMLENRAMVRRKGFGILIGVYGSSVIYMVQLPIFGVIDTPCWIIKAAPSCSEKDGNYACLLREDQGWYCKNAGSTVYYPNDKDCETRGDHVFCDTAAGINVAEQSRECNINISTTNYPCKVSTGRHPISMVALSPLGALVACYKGVSCSIGSNRVGIIKQLPKGCSYITNQDADTVTIDNTVYQLSKVEGEQHVIKGRPVSSSFDPIKFPEDQFNVALDQVFESIENSQALVDQSNKILNSAEKGNTGLVPR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition H: TYR59 ASP62 ARG87 GLU89 TYR100 TYR101 SER102 GLY103 L: ASP1 GLN27 THR28 THR30 ILE31 TRP32 TYR49 ASP50 ALA51 VAL52 SER53 LEU54 GLU55 SER56 SER60 ARG63 GLY64 SER65 GLY66 SER67 TYR91 ASN92 ASN93 A: LEU73 GLN195 ARG198 LEU201 ASN202 ARG205 GLN206 ASP209 ASN210 PRO215 ALA216 ILE217 SER218 LEU219 ASP220 ASP224 ARG248 ALA249 ARG252 ARG253 LYS324 GLU327 ARG329 GLY330 ASN395 ARG396 SER428 VAL430 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
