Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 643 |
| PDBID: | 3RKD |
| Chains: | HL_A |
| Organism: | Paslahepevirus balayani, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 1.90 |
| Reference: | 10.1073/pnas.1101309108 |
| Antibody | |
| Antibody: | 8C11 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Hepatitis E Virus E2s domain (Genotype I) Capsid protein |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >3RKD_H|Chain D, F[auth H]|Monoclonal Antibody, Heavy Chain|Mus musculus (10090) QVTLKESGPGILQPSQTLSLTCSFSGFSLSTSGMGVGWIRQPSGKGLEWLAHIWWDDVKRYSPALKSRLTISKDTSSSQLFLKIASVDTADTATYYCARIKSVITTGDYALDYWGQGTSVAVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDCTSKP |
Light Chain: L
Mutation: NULL
| >3RKD_L|Chain C, E[auth L]|Monoclonal Antibody, Light Chain|Mus musculus (10090) DIQMTQSPASLSVSVGETVTITCRASEIIYSNLAWYQQKQGKSPQLLVYSATNLAEGVPSRFSGSGSGTQYSLKINSLQSEDFGSYYCQHFWGNPWTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: A
Mutation: Y74H
| >3RKD_A|Chain A, B|Capsid protein|Hepatitis E virus (1678143) SRPFSVLRANDVLWLSLTAAEYDQSTYGSSTGPVYVSDSVTLVNVATGAQAVARSLDWTKVTLDGRPLSTIQQHSKTFFVLPLRGKLSFWEAGTTKAGYPYNYNTTASDQLLVENAAGHRVAISTYTTSLGAGPVSISAVAVLAPP |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: ILE2 TYR30 ASN32 PHE91 TRP92 GLY93 ASN94 TRP96 H: SER32 GLY33 TRP54 TRP55 ASP56 VAL58 LYS59 ARG60 TYR61 PRO63 LYS66 SER102 VAL103 ILE104 THR105 THR106 GLY107 TYR109 A: THR476 ALA477 GLU479 ASP481 THR484 TYR485 VAL494 SER495 ASP496 SER497 THR499 GLN508 VAL510 ARG512 SER513 LEU514 ASP515 LYS534 GLU572 ASN573 ALA574 ALA575 GLY576 HIS577 ARG578 PRO592 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
