Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6421 |
| PDBID: | 8OXV |
| Chains: | BC_A |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 1.80 |
| Reference: | 10.1038/s41467-023-42004-z |
| Antibody | |
| Antibody: | DH63-B02 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human transglutaminase 3 |
| Antigen mutation: | No |
| Durg Target: | Q08188 |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >8OXV_B|Chain B|Antibody Fab fragment Heavy chain|Homo sapiens (9606) EVQLVESGGGLVQPGRSLRLSCTASGFTFDDYAMHWVRQAPGKGLEWVSRISWNSRSIAYADSVKGRFTISRDSAKNSLYLQMNSLRTEDTALYYCAKDHYLGSDSYGMDVWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSC |
Light Chain: C
Mutation: NULL
| >8OXV_C|Chain C|Antibody Fab fragment light chain|Homo sapiens (9606) NFMLTQPHSVSESPGKTVTISCTRSSGSIDSNYVQWYQQRPGSAPTIVIHEDNQRPSGVPDRFSGSIDTSSNSASLTISGLKTEDEADYYCQSYDPSNVVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: A
Mutation: NULL
| >8OXV_A|Chain A|Protein-glutamine gamma-glutamyltransferase E 27 kDa non-catalytic chain|Homo sapiens (9606) MAALGVQSINWQTAFNRQAHHTDKFSSQELILRRGQNFQVLMIMNKGLGSNERLEFIVSTGPYPSESAMTKAVFPLSNGSSGGWSAVLQASNGNTLTISISSPASAPIGRYTMALQIFSQGGISSVKLGTFILLFNPWLNVDSVFMGNHAEREEYVQEDAGIIFVGSTNRIGMIGWNFGQFEEDILSICLSILDRSLNFRRDAATDVASRNDPKYVGRVLSAMINSNDDNGVLAGNWSGTYTGGRDPRSWNGSVEILKNWKKSGFSPVRYGQCWVFAGTLNTALRSLGIPSRVITNFNSAHDTDRNLSVDVYYDPMGNPLDKGSDSVWNFHVWNEGWFVRSDLGPSYGGWQVLDATPQERSQGVFQCGPASVIGVREGDVQLNFDMPFIFAEVNADRITWLYDNTTGKQWKNSVNSHTIGRYISTKAVGSNARMDVTDKYKYPEGSDQERQVFQKALGKLKPNTPFAATSSMGLETEEQEPSIIGKLKVAGMLAVGKEVNLVLLLKNLSRDTKTVTVNMTAWTIIYNGTLVHEVWKDSATMSLDPEEEAEHPIKISYAQYEKYLKSDNMIRITAVCKVPDESEVVVERDIILDNPTLTLEVLNEARVRKPVNVQMLFSNPLDEPVRDCVLMVEGSGLLLGNLKIDVPTLGPKEGSRVRFDILPSRSGTKQLLADFSCNKFPAIKAMLSIDVAE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition B: GLY26 PHE27 THR28 ASP30 ASP31 TRP53 HIS100 TYR101 LEU102 GLY103 SER104 ASP105 SER106 C: ASP30 SER31 ASN32 TYR33 HIS50 GLU51 ASP52 ASN53 GLN54 ARG55 SER57 TYR94 PRO96 A: GLU183 ASP184 THR242 GLY243 GLY244 ARG245 TRP250 GLU255 LYS258 ASN259 LYS261 LYS262 SER263 GLY264 SER266 PRO267 VAL268 ARG269 TYR270 ASP627 LEU639 GLY640 ASN641 LEU642 LYS643 ILE644 ASP645 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
