Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6420 |
| PDBID: | 8OL9 |
| Chains: | BA_H |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.60 |
| Reference: | 10.1002/pro.4726 |
| Antibody | |
| Antibody: | NN-8955 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human coagulation factor IX heavy chain |
| Antigen mutation: | No |
| Durg Target: | P00740 |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >8OL9_B|Chain B|Heavy chain of FIXa binding Fab|Homo sapiens (9606) EVQLVESGGGLVQPGRSLRLSCAASGFIFDDYAMHWVRQVPGKGLEWVSGISWNSDNIGYVDSVKGRFTISRDNAKNSLYLQMNSLRIEDTALYYCAKAFGSGSYYNGFDYWGQGALVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSC |
Light Chain: A
Mutation: NULL
| >8OL9_A|Chain A|Light chain of FIXa binding Fab|Homo sapiens (9606) DIQMTQSPSTLSASAGDRVTISCRASQSISSWLAWYQQKPGKAPKLLIYKASSLESGVPSRFSGSGSGTEFTLTISSLQPDDFATYYCQEYNSYIRTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: H
Mutation: NULL
| >8OL9_H|Chain C[auth H]|Coagulation factor IX heavy chain|Homo sapiens (9606) VVGGEDAKPGQFPWQVVLNGKVDAFCGGSIVNEKWIVTAAHCVETGVKITVVAGEHNIEETEHTEQKRNVIRIIPHHNYNAAINKYNHDIALLELDEPLVLNSYVTPICIADKEYTNIFLKFGSGYVSGWGRVFHKGRSALVLQYLRVPLVDRATCLRSTKFTIYNNMFCAGFHEGGRDSCQGDSGGPHVTEVEGTSFLTGIISWGEECAMKGKYGIYTKVSRYVNWIKEKTKLT |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: TRP32 TYR91 ASN92 B: ASP31 SER52 TRP53 ASP56 ASN57 SER102 GLY103 SER104 TYR105 TYR106 ASN107 H: ARG207 ALA208 THR209 LEU211 ARG212 SER213 THR214 LYS215 PHE216 THR217 TYR219 LYS268 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
