Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6410 |
| PDBID: | 8IVA |
| Chains: | HL_G |
| Organism: | Homo sapiens, Severe acute respiratory syndrome coronavirus 2, Mus musculus |
| Method: | EM |
| Resolution (Å): | 3.95 |
| Reference: | 10.1093/procel/pwad040 |
| Antibody | |
| Antibody: | 3E2 Fv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | SARS-CoV-2 spike glycoprotein RBD |
| Antigen mutation: | No |
| Durg Target: | P0DTC2; P0DTC2; |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >8IVA_H|Chain E[auth H]|heavy chain of 3E2|Mus musculus (10090) EVMLVESGGGVVKPGGSLKLSCAASGFSFSTYAMSWIRQTPEKSLEWVAAISSGGTNTYYPGSVKGRFTISRDKAMNTLYLQLSSLRSEDTAMYYCVRHSGNYVDSVMDYWGQGTSVTVSS |
Light Chain: L
Mutation: NULL
| >8IVA_L|Chain D[auth L]|light chain of 3E2|Mus musculus (10090) QIVLTQSPAIMSASLGEEITLTCSVSSSVSDMHWYQQKSGTSPKVFIYSTSNLASGVPSRFSGSGSGTFYSLTISSVEAEDAAYYYCHQWSSWTFGGGTKLEIK |
Antigen
Chain: G
Mutation: NULL
| >8IVA_G|Chain C[auth G]|Spike protein S1|Severe acute respiratory syndrome coronavirus 2 (2697049) ESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGP |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: GLN1 ILE2 SER27 SER28 VAL29 SER30 ASP31 SER49 GLN89 TRP90 SER91 SER92 TRP93 H: TRP47 THR56 ASN57 TYR59 TYR60 GLY62 LYS65 ASN102 TYR103 VAL104 ASP105 G: ALA372 SER373 PHE374 SER375 THR376 ARG403 GLY404 ASP405 GLU406 ARG408 GLN409 ASN437 ASN439 ASN440 GLN498 PRO499 THR500 ASN501 GLY502 VAL503 GLY504 TYR505 GLN506 TYR508 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
