Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6408 |
| PDBID: | 8IV8 |
| Chains: | CD_G |
| Organism: | Mus musculus, Severe acute respiratory syndrome coronavirus 2 |
| Method: | EM |
| Resolution (Å): | 3.92 |
| Reference: | 10.1093/procel/pwad040 |
| Antibody | |
| Antibody: | 1C4 Fv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | SARS-CoV-2 spike glycoprotein RBD |
| Antigen mutation: | No |
| Durg Target: | P0DTC2; P0DTC2; |
Sequence information
Antibody
Heavy Chain: C
Mutation: NULL
| >8IV8_C|Chain E[auth C]|heavy chain of 1C4|Mus musculus (10090) QIQLVQSGPELKKPGETVKISCKASGYTFTDYGLNWVKQAPGKGLKWMGWINTYSGEPTYNDEFRGRFAFSLETSTITAYLKINNLKNEDTATYFCARGGNWDWYFDVWGAGTTVTVSS |
Light Chain: D
Mutation: NULL
| >8IV8_D|Chain D|light chain of 1C4|Mus musculus (10090) DIVLTQSPATLSVTPGDNVSLSCRASQIISNNLHWYQQKSHESPRLLIKYASQSISGIPSRFSGSGSGTDFTLSINSVETEDFGMYFCQQSNTWPLTCGSGTKLELN |
Antigen
Chain: G
Mutation: NULL
| >8IV8_G|Chain C[auth G]|Spike protein S1|Severe acute respiratory syndrome coronavirus 2 (2697049) ESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGP |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: GLN27 ILE28 ASN32 TYR50 SER91 ASN92 THR93 TRP94 LEU96 C: THR30 ASP31 TRP50 ASN52 TYR54 SER55 GLU57 THR59 ASN101 TRP102 ASP103 TRP104 G: GLY339 GLU340 ASN343 ALA344 THR345 ARG346 ASN439 ASN440 LEU441 ASP442 SER443 LYS444 VAL445 GLY446 ASN448 ASN450 TYR451 PRO499 THR500 ARG509 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
