Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6405 |
| PDBID: | 8IV5 |
| Chains: | HL_G |
| Organism: | Severe acute respiratory syndrome coronavirus 2, Mus musculus |
| Method: | EM |
| Resolution (Å): | 3.77 |
| Reference: | 10.1093/procel/pwad040 |
| Antibody | |
| Antibody: | 8H12 Fv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | SARS-CoV-2 spike glycoprotein RBD |
| Antigen mutation: | No |
| Durg Target: | P0DTC2; P0DTC2; |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >8IV5_H|Chain C[auth H]|heavy chain of 8H12|Mus musculus (10090) EVKLEESGGGLVQPGGSMKLSCAASGFTFSDAWMDWVRQSPEKGLEWVAQIRRKANNHATYYAESVKGRFTISRDDSKSSVYLQMNSLRAEDTGIYYCIRGMTYAMDFWGQGTSVTVSS |
Light Chain: L
Mutation: NULL
| >8IV5_L|Chain B[auth L]|light chain of 8H12|Mus musculus (10090) DIVMTQFQKFMSTSVGDRVSITCKASQNVRTAVAWYQQKPGQSPKAMIYLASNRHRGVPDRFTGSGCGTDFTLTISNVQCEDLADYFCLQHRNYPLTFGGGTKLEIK |
Antigen
Chain: G
Mutation: NULL
| >8IV5_G|Chain A[auth G]|Spike protein S1|Severe acute respiratory syndrome coronavirus 2 (2697049) ESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGP |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: ARG30 THR31 ALA32 TYR49 LEU50 SER52 ASN53 ARG54 ARG56 ASP60 HIS91 ARG92 ASN93 TYR94 LEU96 H: TRP33 GLN50 ARG52 ARG53 ALA55 ASN56 MET102 THR103 TYR104 G: ARG403 LYS417 LEU455 PHE456 TYR473 ALA475 GLY476 SER477 THR478 CYS480 VAL483 GLU484 GLY485 PHE486 ASN487 TYR489 PHE490 GLN493 SER494 TYR495 GLY496 TYR505 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
