Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6403 |
| PDBID: | 8IV4 |
| Chains: | AB_G |
| Organism: | Mus musculus, Severe acute respiratory syndrome coronavirus 2 |
| Method: | EM |
| Resolution (Å): | 3.59 |
| Reference: | 10.1093/procel/pwad040 |
| Antibody | |
| Antibody: | 8H12 Fv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | SARS-CoV-2 spike glycoprotein RBD |
| Antigen mutation: | No |
| Durg Target: | P0DTC2; P0DTC2; |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >8IV4_A|Chain D[auth A]|heavy chain of 8H12|Mus musculus (10090) EVKLEESGGGLVQPGGSMKLSCAASGFTFSDAWMDWVRQSPEKGLEWVAQIRRKANNHATYYAESVKGRFTISRDDSKSSVYLQMNSLRAEDTGIYYCIRGMTYAMDFWGQGTSVTVSS |
Light Chain: B
Mutation: NULL
| >8IV4_B|Chain C[auth B]|light chain of 8H12|Mus musculus (10090) DIVMTQFQKFMSTSVGDRVSITCKASQNVRTAVAWYQQKPGQSPKAMIYLASNRHRGVPDRFTGSGCGTDFTLTISNVQCEDLADYFCLQHRNYPLTFGGGTKLEIK |
Antigen
Chain: G
Mutation: NULL
| >8IV4_G|Chain E[auth G]|Spike protein S1|Severe acute respiratory syndrome coronavirus 2 (2697049) ESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGP |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: ARG30 THR31 ALA32 LEU50 ASN53 ARG56 HIS91 ARG92 ASN93 TYR94 LEU96 A: TRP33 ASP35 GLN50 ARG52 ARG53 ALA55 ASN56 MET102 THR103 TYR104 G: LYS417 LEU455 PHE456 TYR473 ALA475 GLY476 SER477 THR478 VAL483 GLU484 GLY485 PHE486 ASN487 TYR489 PHE490 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
