Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6393 |
| PDBID: | 8HS2 |
| Chains: | BC_R |
| Organism: | Rattus, Escherichia coli, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.08 |
| Reference: | 10.1038/s41421-023-00520-8 |
| Antibody | |
| Antibody: | Ab046 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | G-protein coupled receptor 20 (GPR20) |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >8HS2_B|Chain A[auth B]|heavy chain of Fab046|Rattus (10114) MEWNWVFLFLLSVTAEVHSQVQLQQSGAELAKPGSSVKISCKASGYTFTSYYISWIKQTTGQGLKYIGFINPGSGHTNYNEKFKGKATLTVDKSSSTAFMQLSSLTPDDSAIYYCARGAGGFLRIITKFDYWGQGVMVTVSSAQTTAPSVYPLAPGCGDTTSSTVTLGCLVKGYFPEPVTVTWNSGALSSDVHTFPAVLQSGLYTLTSSVTSSTWPSQTVTCNVAHPASSTKVDKKVGGSGHHHHHH |
Light Chain: C
Mutation: NULL
| >8HS2_C|Chain B[auth C]|Light chain of Fab046|Rattus (10114) METDRLLLWVLLLWVPGSTGDTVLTQSPALAVSLGQRVTISCRASKSVSTYIHWYQQRSGQQPKLLIYSASNLESGVPSRFSGSGSGTDFTLTIDPVEPDDIANYYCQQINELPYTFGAGTKLELKRADAAPTVSIFPPSTERLATGGASVVCLMNNFYPRDISVKWKIDGTERRDGVLDSVTDQDSKDSTYSMSSTLSLTKADYESHNLYTCEVVHKTSSSPVVKSFNRNEC |
Antigen
Chain: R
Mutation: L272W/D426N
| >8HS2_R|Chain C[auth R]|Soluble cytochrome b562,G-protein coupled receptor 20|Escherichia coli (562) MKTIIALSYIFCLVFADYKDDDDAGRAADLEDNWETLNDNLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLKTTRNAYIQKYLMPSVSPAGPSAGAVPNATAVTTVRTNASGLEVPLFHLFARLDEELHGTFPGLWLALMAVHGAIFLAGLVLNGLALYVFCCRTRAKTPSVIYTINLVVTDLLVGLSLPTRFAVYYGARGCLRCAFPHVLGYFLNMHCSIWFLTCICVDRYLAIVRPEGSRRCRQPACARAVCAFVWLAAGAVTLSVLGVTGSRPCCRVFALTVLEFLLPLLVISVFTGRIMCALSRPGLLHQGRQRRVRAMQLLLTVLIIFLVCFTPFHARQVAVALWPDMPHHTSLVVYHVAVTLSSLNSCMNPIVYCFVTSGFQATVRGLFGQHGHHHHHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: THR49 SER50 TYR52 ASN71 GLY73 SER74 GLY75 HIS76 ASN78 LYS93 GLY120 GLY121 PHE122 LEU123 ARG124 ILE125 C: ASN378 LEU380 R: HIS36 ALA39 ARG40 GLU43 GLU44 HIS46 GLY47 THR48 PRO50 LEU54 MET57 TYR113 TYR114 ARG117 GLY118 CYS119 LEU120 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
