Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 639 |
| PDBID: | 3R08 |
| Chains: | HL_E |
| Organism: | Nothocricetulus migratorius |
| Method: | XRD |
| Resolution (Å): | 4.10 |
| Reference: | 10.1074/jbc.M111.332783 |
| Antibody | |
| Antibody: | 2C11 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | T cell surface glycoprotein CD3 epsilon chain |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >3R08_H|Chain B[auth H]|Mouse anti-mouse CD3epsilon antibody 2C11 heavy chain|Cricetulus migratorius (3122392) EVQLVESGGGLVQPGKSLKLSCEASGFTFSGYGMHWVRQAPGRGLESVAYITSSSINIKYADAVKGRFTVSRDNAKNLLFLQMNILKSEDTAMYYCARFDWDKNYWGQGTMVTVSSAKTTAPSVYPLAPACDSTTSTTNTVTLGCLVKGYFPEPVTVIWNSGALTSGVHTFPSVLHSGLYSLSSSVTVPSSTWPSQTVTCNVAHPASSTTVDLKIE |
Light Chain: L
Mutation: NULL
| >3R08_L|Chain A[auth L]|Mouse anti-mouse CD3epsilon antibody 2C11 light chain|Cricetulus migratorius (3122392) DIQMTQSPSSLPASLGDRVTINCQASQDISNYLNWYQQKPGKAPKLLIYYTNKLADGVPSRFSGSGSGRDSSFTISSLESEDIGSYYCQQYYNYPWTFGPGTKLEIKRADAKPTVSIFPPSSEQLGTGSATLVCFVNNFYPKDINVKWKVDGSEKRDGVLQSVTDQDSKDSTYSLSSTLSLTKADYERHNLYTCEVTHKTSTAAIVKTLNRNE |
Antigen
Chain: E
Mutation: NULL
| >3R08_E|Chain C[auth E]|T-cell surface glycoprotein CD3 epsilon chain|Cricetulus migratorius (3122392) DDAENIEYKVSISGTSVELTCPLDSDENLKWEKNGQELPQKHDKHLVLQDFSEVEDSGYYVCYTPASNKNTYLYLKARVSEY |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition L: TYR32 TYR91 TYR92 ASN93 TYR94 TRP96 H: THR28 GLY31 TYR32 GLY33 HIS35 TYR50 SER52 SER53 ILE55 ASN56 ILE57 LYS58 TYR59 LYS64 GLY65 PHE95 ASP96 TRP97 E: ASP1 ASP2 ALA3 GLU4 ASN5 LEU23 ASP24 SER25 ASP26 GLU27 ASN28 LYS30 TYR63 THR64 PRO65 ALA66 SER67 ASN68 LYS69 ASN70 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
