Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6386 |
| PDBID: | 8HII |
| Chains: | HL_A |
| Organism: | Escherichia coli, Homo sapiens, Lama glama |
| Method: | EM |
| Resolution (Å): | 3.57 |
| Reference: | 10.1038/s41421-022-00508-w |
| Antibody | |
| Antibody: | Anti-BRIL Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | BRIL-SLC19A1 chimera |
| Antigen mutation: | No |
| Durg Target: | P0ABE7 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >8HII_H|Chain B[auth H]|anti-BRIL Fab heavy chain|Homo sapiens (9606) MGWSLILLFLVAVATRVLSEISEVQLVESGGGLVQPGGSLRLSCAASGFNVVDFSLHWVRQAPGKGLEWVAYISSSSGSTSYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARWGYWPGEPWWKAFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKSNSLEVLFQ |
Light Chain: L
Mutation: NULL
| >8HII_L|Chain D[auth L]|anti-BRIL Fab light chain|Homo sapiens (9606) MGWSCIILFLVATATGVHSSDIQMTQSPSSLSASVGDRVTITCRASQSVSSAVAWYQQKPGKAPKLLIYSASSLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQYLYYSLVTFGQGTKVEIKRTVAAPSVFIFPPSDSQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >8HII_A|Chain A|BRIL-SLC19A1 chimera|Escherichia coli (562) GPSRATADLEDNWETLNDNLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLKTTRNAYIQKYLSWRHLVCYLCFYGFMAQIRPGESFITPYLLGPDKNFTREQVTNEITPVLSYSYLAVLVPVFLLTDYLRYTPVLLLQGLSFVSVWLLLLLGHSVAHMQLMELFYSVTMAARIAYSSYIFSLVRPARYQRVAGYSRAAVLLGVFTSSVLGQLLVTVGRVSFSTLNYISLAFLTFSVVLALFLKRPKRSLFFNRDDRGRCETSASELERMNPGPGGKLGHALRVACGDSVLARMLRELGDSLRRPQLRLWSLWWVFNSAGYYLVVYYVHILWNEVDPTTNSARVYNGAADAASTLLGAITSFAAGFVKIRWARWSKLLIAGVTATQAGLVFLLAHTRHPSSIWLCYAAFVLFRGSYQFLVPIATFQIASSLSKELCALVFGVNTFFATIVKTIITFIVSDVRGLGLPVRKQFQLYSVYFLILSIIYFLGAMLDGLRHCQRGHHPRQPPAQGLRSAAEEKAAQALSVQDKGLGGLQPAQSPPLSPEDSLGAVGPASLEQRQSDPYLAQAPAPQAAEFLSPVTTPSPCTLCSAQASGPEAADETCPQLAVHPPGVSKLGLQCLPSDGVQNVNQSNS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: SER58 GLY59 SER60 SER62 TRP105 PRO106 TRP110 TRP111 L: ASP2 GLN28 SER29 VAL30 SER31 SER32 ALA33 ARG67 LEU93 TYR94 TYR95 SER96 LEU97 A: ASN-77 LYS-56 ASP-55 THR-52 LYS-51 ARG-49 ALA-48 LEU-45 ASP-44 GLN-42 LYS-41 ASP-17 VAL-14 GLY-13 ASP-10 LEU-7 LYS-6 ASN-3 GLU-2 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
