Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 637 |
| PDBID: | 3QUM |
| Chains: | BA_P |
| Organism: | Homo sapiens, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 3.20 |
| Reference: | 10.1016/j.jmb.2011.10.007 |
| Antibody | |
| Antibody: | 5D5A5 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Prostate-specific antigen |
| Antigen mutation: | No |
| Durg Target: | P07288; P07288; |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >3QUM_B|Chain E[auth B], J[auth D]|Fab 5D5A5 Heavy Chain|Mus musculus (10090) QVQLQQSGAELAKPGASVKMSCKTSGYSFSSYWMHWVKQRPGQGLEWIGYINPSTGYTENNQKFKDKVTLTADKSSNTAYMQLNSLTSEDSAVYYCARSGRLYFDVWGAGTTVTVSSAKTTAPPVYPLAPVCGDTTGSSVTLGCLVKGYFPESVTLLWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIVPRDC |
Light Chain: A
Mutation: NULL
| >3QUM_A|Chain D[auth A], I[auth C]|Fab 5D5A5 Light Chain|Mus musculus (10090) DIVLTQSPPSLAVSLGQRATISCRASESIDLYGFTFMHWYQQKPGQPPKILIYRASNLESGIPARFSGSGSRTDFTLTINPVEADDVATYYCQQTHEDPYTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: P
Mutation: NULL
| >3QUM_P|Chain A[auth P], F[auth Q]|Prostate-specific antigen|Homo sapiens (9606) IVGGWECEKHSQPWQVLVASRGRAVCGGVLVHPQWVLTAAHCIRNKSVILLGRHSLFHPEDTGQVFQVSHSFPHPLYDMSLLKNRFLRPGDDSSHDLMLLRLSEPAELTDAVKVMDLPTQEPALGTTCYASGWGSIEPEEFLTPKKLQCVDLHVISNDVCAQVHPQKVTKFMLCAGRWTGGKSTCSGDSGGPLVCNGVLQGITSWGSEPCALPERPSLYTKVVHYRKWIKDTIVANP |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition A: LEU27 TYR28 GLY29 PHE30 PHE32 ARG50 HIS92 GLU93 ASP94 TYR96 B: SER30 SER31 TYR32 TRP33 TRP47 TYR50 SER53 THR54 TYR56 GLU58 ARG94 SER95 GLY96 ARG97 LEU98 ASP102 P: LEU32 ALA34 ARG36 GLY37 ARG38 ALA39 ASN61 LYS62 SER63 VAL64 LEU66 ARG69 LEU72 PHE73 PRO76 GLU77 GLY80 GLN81 VAL82 PHE83 GLN84 VAL85 SER86 HIS87 ARG107 SER109 GLU110 PHE149 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
