Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6341 |
| PDBID: | 8H64 |
| Chains: | B_A |
| Organism: | Listeria monocytogenes EGD-e, Lama glama |
| Method: | XRD |
| Resolution (Å): | 2.35 |
| Reference: | 10.1016/j.jbc.2023.105254 |
| Antibody | |
| Antibody: | 24 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | The Listeria monocytogenes virulence factor, internalin family protein InlA |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >8H64_B|Chain B, D, F, H|Anti-internalin A VHH24|Lama glama (9844) EVQLVESGGGLVQPGGSLRLSCAASGFTLDRYAIGWFRQAPGKEREGVSVISDTITAYRNSVKGRFTISRDNAKNTVYLQMNRLKPEDTAIYYCAVRDRGRVYSSTWSGFGSWGQGTQVTISS |
Antigen
Chain: A
Mutation: NULL
| >8H64_A|Chain A, C, E, G|Internalin A|Listeria monocytogenes serovar 1/2a (169963) SATITQDTPINQIFTDTALAEKMKTVLGKTNVTDTVSQTDLDQVTTLQADRLGIKSIDGVEYLNNLTQINFSNNQLTDITPLKNLTKLVDILMNNNQIADITPLANLTNLTGLTLFNNQITDIDPLKNLTNLNRLELSSNTISDISALSGLTSLQQLSFGNQVTDLKPLANLTTLERLDISSNKVSDISVLAKLTNLESLIATNNQISDITPLGILTNLDELSLNGNQLKDIGTLASLTNLTDLDLANNQISNLAPLSGLTKLTELKLGANQISNISPLAGLTALTNLELNENQLEDISPISNLKNLTYLTLYFNNISDISPVSSLTKLQRLFFYNNKVSDVSSLANLTNINWLSAGHNQISDLTPLANLTRITQLGLNDQAWTNAPVNYKANVSIPNTVKNVTGALIAPATISDGGSYTEPDITWNLPSYTNEVSYTFSQPVTIGKGTTTFSGTVTQPLKA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: ASP30 ARG31 ALA33 VAL50 SER52 ASP53 ILE55 THR56 ALA57 TYR58 ARG59 ASN60 LYS63 ARG97 ASP98 ARG99 GLY100 ARG101 VAL102 TYR103 SER104 SER105 THR106 TRP107 A: ASN238 ASN239 GLN240 ASN259 GLY260 ASN261 GLN262 ALA281 ASN282 ASN283 ALA304 ASN305 GLN306 GLU323 ASN325 GLU326 ASN327 GLN328 THR345 TYR347 PHE348 ASN349 ASN350 ARG365 PHE367 TYR369 ASN370 TRP387 SER389 HIS392 ASN413 ASP414 GLN415 ALA416 THR472 PHE473 SER487 GLY488 THR489 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
