Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6340 |
| PDBID: | 8H63 |
| Chains: | B_A |
| Organism: | Listeria monocytogenes EGD-e, Lama glama |
| Method: | XRD |
| Resolution (Å): | 1.53 |
| Reference: | 10.1016/j.jbc.2023.105254 |
| Antibody | |
| Antibody: | 10 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | The Listeria monocytogenes virulence factor, internalin family protein InlA |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >8H63_B|Chain B|VHH10|Lama glama (9844) ELQLVESGGGLVQAGGSLTVSCAASGSAFSVNVMGWSRQAPGKERELVAGITRRGNTYYADTVKGRFTISRDNAKNTLYLQMNSLKPEDTAMYYCAALADIATMGPNDYWGQGTQVTVSG |
Antigen
Chain: A
Mutation: NULL
| >8H63_A|Chain A|Internalin A|Listeria monocytogenes serovar 1/2a (169963) SATITQDTPINQIFTDTALAEKMKTVLGKTNVTDTVSQTDLDQVTTLQADRLGIKSIDGVEYLNNLTQINFSNNQLTDITPLKNLTKLVDILMNNNQIADITPLANLTNLTGLTLFNNQITDIDPLKNLTNLNRLELSSNTISDISALSGLTSLQQLSFGNQVTDLKPLANLTTLERLDISSNKVSDISVLAKLTNLESLIATNNQISDITPLGILTNLDELSLNGNQLKDIGTLASLTNLTDLDLANNQISNLAPLSGLTKLTELKLGANQISNISPLAGLTALTNLELNENQLEDISPISNLKNLTYLTLYFNNISDISPVSSLTKLQRLFFYNNKVSDVSSLANLTNINWLSAGHNQISDLTPLANLTRITQLGLNDQAWTNAPVNYKANVSIPNTVKNVTGALIAPATISDGGSYTEPDITWNLPSYTNEVSYTFSQPVTIGKGTTTFSGTVTQPLKA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: GLN3 SER25 GLY26 SER27 SER30 VAL31 VAL33 THR52 ARG53 ARG54 ASN56 THR57 TYR58 TYR59 ASP61 LYS64 ASN73 ALA99 ASP100 ILE101 ALA102 THR103 MET104 GLY105 PRO106 TYR109 A: ARG85 ASN129 ASN151 SER172 SER173 SER215 SER216 THR237 ASN238 ASN239 ASN259 GLY260 ASN261 ASN282 ASN283 ALA304 ASN305 GLN306 GLU326 THR345 TYR347 PHE348 ARG365 PHE367 TYR369 TRP387 SER389 HIS392 THR408 GLN409 LEU410 GLY411 ASN413 ASP414 SER474 THR485 SER487 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
