Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 632 |
| PDBID: | 3QA3 |
| Chains: | HF_I |
| Organism: | Mus musculus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.00 |
| Reference: | 10.4049/jimmunol.1102394 |
| Antibody | |
| Antibody: | 107 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Integrin alpha-M |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >3QA3_H|Chain B, E[auth D], H, K|Antibody Heavy chain|Mus musculus (10090) QVQLQQSGAELVKPGASVKLSCTPSGFNIKDIYMQWVKQRPEQGLEWIGRIDPANDKTKYDPKFQGKATITADTSSNTAYLQLSSLTSEDTAVYYCASEGHYGYDGYAMDYWGQGTTVTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSPRPSETVTCNVAHPASSTKVDKKIVPRDC |
Light Chain: F
Mutation: NULL
| >3QA3_F|Chain A, D[auth C], G[auth F], J|Antibody Light chain|Mus musculus (10090) DIEMTQSPSSLGVSVGEKVTMSCKSSQNLLYSSNQKNYLAWYQQKPGQSPKLLIYWASTRESGVPDRFTGTGSGTDFTLTISSVKAEDLAVYYCQQYYSYPLTFGAGTKLELKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: I
Mutation: I185G
| >3QA3_I|Chain C[auth E], F[auth G], I, L|Integrin alpha-M|Homo sapiens (9606) DSDIAFLIDGSGSIIPHDFRRMKEFVSTVMEQLKKSKTLFSLMQYSEEFRIHFTFKEFQNNPNPRSLVKPITQLLGRTHTATGIRKVVRELFNITNGARKNAFKILVVITDGEKFGDPLGYEDVIPEADREGVIRYVIGVGDAFRSEKSRQELNTIASKPPRDHVFQVNNFEALKTIQNQLREKGFAIEG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition F: TYR31 SER32 SER33 TYR38 TYR97 TYR98 SER99 TYR100 LEU102 H: TYR35 ARG52 ASP54 ASN57 LYS59 THR60 LYS61 GLU101 HIS103 GLY105 TYR106 ASP107 GLY108 I: SER142 GLY143 SER144 ILE146 GLU178 GLU179 PHE180 ARG181 LEU205 LEU206 GLY207 ARG208 THR209 HIS210 ASP242 GLY243 GLU244 PHE246 GLY247 ALA274 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
