Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6308 |
| PDBID: | 8GV5 |
| Chains: | CD_B |
| Organism: | Influenza A virus (A/swine/Guangdong/104/2013(H1N1)), Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.20 |
| Reference: | 10.1038/s41467-022-35236-y |
| Antibody | |
| Antibody: | PN-SIA28 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Influenza A virus swine/Guangdong/104/2013 H1N1 hemagglutinin HA2 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: C
Mutation: NULL
| >8GV5_C|Chain C|PN-SIA28 Heavy chain|Homo sapiens (9606) QVQLVESGGGVVQPGRSLRLSCAASGFPFSSYGMHWVRQAPGKGLEWVAGVSYDGSYKYYADSVKGRFTISRDSSKSTLYLQMNSLRPEDTAVYYCARPSAIFGIYIILNGLDVWGQGTTVTVSS |
Light Chain: D
Mutation: NULL
| >8GV5_D|Chain D|PN-SIA28 Light Chain|Homo sapiens (9606) EIVLTQSPSSVSASVGDRVTITCRATQGISSWLAWYQQKPGKPPKLLIFGASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYFCQQAHSFPLTFGGGTKVEIKR |
Antigen
Chain: B
Mutation: NULL
| >8GV5_B|Chain B|Hemagglutinin HA2 chain|Influenza A virus (A/swine/Guangdong/104/2013(H1N1)) (1490471) GAIAGFIEGGWTGMIDGWYGYHHQNEHGSGYAADQKSTQIAIDGIRNKVNSVIEKMNIQFTSVGKEFNSLEKRMENLNKKVDDGFLDVWTYNAELLILLENERTLDYHDFNVKNLYEKVKSQLRNNAKEIGNGCFEFYHKCDNECMESVKNGTYNYPKYSEESKLNREEIDGV |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: SER61 SER82 TYR83 ASP84 TYR87 TYR89 ILE132 PHE133 GLY134 ILE135 TYR136 ILE137 ILE138 LEU139 ASN140 D: SER72 SER73 TRP74 PHE91 GLY92 SER94 SER95 ALA133 HIS134 B: GLY343 MET344 ILE345 ASP346 GLY347 TRP348 HIS353 TYR361 ALA362 GLN365 LYS366 THR368 GLN369 ILE372 ASP373 ILE375 ARG376 VAL379 ASN380 GLU384 GLU477 LYS480 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
