Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 63 |
| PDBID: | 1IQD |
| Chains: | BA_C |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.00 |
| Reference: | 10.1182/blood.v98.1.13 |
| Antibody | |
| Antibody: | BO2C11 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | infused factor VIII C2 Domain |
| Antigen mutation: | No |
| Durg Target: | P00451 |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >1IQD_B|Chain B|HUMAN MONOCLONAL BO2C11 FAB HEAVY CHAIN|Homo sapiens (9606) QVQLVQSGAEVKKPGASVKVSCKVSGYTLTELPVHWVRQAPGKGLEWVGSFDPESGESIYAREFQGSVTMTADTSTNIAYMELSSLRSDDTAVYYCAVPDPDAFDIWGQGTMVTVSSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTATYTCNVDHKPSNTKVDKRV |
Light Chain: A
Mutation: NULL
| >1IQD_A|Chain A|HUMAN MONOCLONAL BO2C11 FAB LIGHT CHAIN|Homo sapiens (9606) IALTQSPGTLSLSPGERATLSCRASQSFSSSYLAWYQQKPGQAPRLLIYGASTRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQKYGTSAITFGQGTRLEIKGTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNR |
Antigen
Chain: C
Mutation: NULL
| >1IQD_C|Chain C|HUMAN FACTOR VIII|Homo sapiens (9606) CSMPLGMESKAISDAQITASSYFTNMFATWSPSKARLHLQGRSNAWRPQVNNPKEWLQVDFQKTMKVTGVTTQGVKSLLTSMYVKEFLISSSQDGHQWTLFFQNGKVKVFQGNQDSFTPVVNCLDPPLLTRYLRIHPQSWVHQIALRMEVLGCEAQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: SER30 SER31 SER32 TYR33 TYR50 GLY51 THR54 ARG55 THR57 TYR92 GLY93 THR94 ILE97 B: GLN1 VAL2 TYR27 THR30 GLU31 LEU32 PRO33 HIS35 SER50 PHE51 ASP52 GLU54 GLU57 ILE59 VAL95 ASP97 PRO98 ASP99 ILE103 C: PHE2196 THR2197 ASN2198 MET2199 PHE2200 ALA2201 TRP2203 ARG2215 ARG2220 GLN2222 VAL2223 ASN2224 SER2250 LEU2251 LEU2252 THR2253 MET2255 HIS2315 GLN2316 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
