Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6252 |
| PDBID: | 8GHP |
| Chains: | HL_A |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.52 |
| Reference: | 10.1038/s41598-023-40467-0 |
| Antibody | |
| Antibody: | anti-GUCY2C Fv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human guanylyl cyclase C |
| Antigen mutation: | No |
| Durg Target: | P25092 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >8GHP_H|Chain B[auth H]|anti-GUCY2C-scFv antibody heavy chain|Homo sapiens (9606) EVQLVESGGGLVQPGGSLRLSCAASGFTFSSYWMHWVRQAPGKGLEWIGEIKPSNELTNVHEKFKDRFTISVDKAKNSAYLQMNSLRAEDTAVYYCTRTITTTEGYWFFDVWGQGTLVTVSS |
Light Chain: L
Mutation: NULL
| >8GHP_L|Chain C[auth L]|anti-GUCY2C-scFv antibody light chain|Homo sapiens (9606) DIQLTQSPSSLSASVGDRVTITCRASESVDYYGSSLLQWYQQKPGKAPKLLIYAASKLASGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQTRKAYTFGQGTKLEIKTGSENLYFQ |
Antigen
Chain: A
Mutation: NULL
| >8GHP_A|Chain A|Guanylyl cyclase C|Homo sapiens (9606) STGSQVSQNCHNGSYEISVLMMGNSAFAEPLKNLEDAVNEGLEIVRGRLQNAGLNVTVNATFMYSDGLIHNSGDCRSSTCEGLDLLRKISNAQRMGCVLIGPSCTYSTFQMYLDTELSYPMISAGSFGLSCDYKETLTRLMSPARKLMYFLVNFWKTNDLPFKTYSWSTSYVYKNGTETEDCFWYLNALEASVSYFSHELGFKVVLRQDKEFQDILMDHNRKSNVIIMCGGPEFLYKLKGDRAVAEDIVIILVDLFNDQYFEDNVTAPDYMKNVLVLTLSPGNSLLNSSFSRNLSPTKRDFALAYLNGILLFGHMLKIFLENGENITTPKFAHAFRNLTFEGYDGPVTLDDWGDVDSTMVLLYTSVDTKKYKVLLTYDTHVNKTYPVDMSPTFTWKNSKLPNDITGRGPQGGGGSENLYFQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: TRP33 GLU50 LYS52 LEU57 ASN59 VAL60 GLU62 LYS65 THR99 THR101 THR103 GLU104 GLY105 TYR106 TRP107 L: TYR31 TYR32 SER34 LEU36 THR95 ARG96 LYS97 ALA98 TYR99 A: CYS72 ARG73 SER74 SER75 THR76 GLU78 GLY79 LEU80 LEU82 LEU83 ARG84 ILE86 SER87 ASN88 ARG91 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
