Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6251 |
| PDBID: | 8GH4 |
| Chains: | HL_E |
| Organism: | Homo sapiens, Bos taurus |
| Method: | XRD |
| Resolution (Å): | 3.80 |
| Reference: | 10.1016/j.biopha.2023.114494 |
| Antibody | |
| Antibody: | 1H5 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Bos taurus disintegrin and metalloproteinase domain-containing protein 10 (ADAM10 disentegrin cysteine rich domains) |
| Antigen mutation: | No |
| Durg Target: | O14672 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >8GH4_H|Chain A[auth H]|Antibody heavy chain|Homo sapiens (9606) QVQLVESGGGLVKPGGSLRLSCAASGFTFSDYYMSWIRQAPGKGLEWVSYISSSGSTIYYADSVKGRFTISRDNAKNSLYLQMNSLRAEDTAVYYCARDFYDSKIFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKT |
Light Chain: L
Mutation: NULL
| >8GH4_L|Chain B[auth L]|Antibody light chain|Homo sapiens (9606) DIQMTQSPSSLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPKLLIYAASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDVGVYYCMEGLKTPFTFGPGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: E
Mutation: NULL
| >8GH4_E|Chain C[auth E]|Disintegrin and metalloproteinase domain-containing protein 10|Bos taurus (9913) SGQPICGNGMVEQGEECDCGYSDQCKDECCYDANQPEGKKCKLKPGKQCSPSQGPCCTAHCAFKSKTEKCRDDSDCAKEGICNGITALCPASDPKPNFTDCNRHTQVCINGQCAGSICEKHGLEECTCASSDGKDDKELCHVCCMKKMEPSTCASTGSVQWNKYFLGRTITLQPGSPCNDFRGYCDVFMRCRASGS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: TYR33 TRP47 TYR50 SER53 SER55 THR56 TYR58 ASP95 PHE96 TYR97 ASP98 SER99 L: TYR32 TYR49 GLY91 LEU92 LYS93 THR94 PRO95 PHE96 E: GLY587 LYS588 ASP590 LYS591 PRO628 GLY629 TYR638 CYS639 ASP640 VAL641 PHE642 MET643 ARG644 CYS645 ARG646 ALA647 SER648 GLY649 SER650 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
