Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6249 |
| PDBID: | 8GF2 |
| Chains: | HL_A |
| Organism: | Severe acute respiratory syndrome coronavirus 2, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.85 |
| Reference: | 10.1126/scisignal.abk3516 |
| Antibody | |
| Antibody: | eCR3022.20 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | SARS-CoV-2 spike glycoprotein RBD |
| Antigen mutation: | No |
| Durg Target: | P0DTC2; P0DTC2; |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >8GF2_H|Chain D[auth H], I[auth X]|eCR3022.20 Fab heavy chain|Homo sapiens (9606) QMQLVQSGTEVKKPGESLKISCKGSGYGFIWYWIGWVRQMPGKGLEWMGIIYPGDGETRYSPSFQGQVTISADKSINTAYLQWSSLKASDTAIYYCAGGGGISTPMDVWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSC |
Light Chain: L
Mutation: NULL
| >8GF2_L|Chain E[auth L], J[auth Y]|eCR3022.20 Fab light chain|Homo sapiens (9606) DIQLTQSPDSLAVSLGERATINCKSSQSVLWSSINKNYLAWYQQKPGQPPKLLIYWASTPESGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQQYYKTPYTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGECS |
Antigen
Chain: A
Mutation: NULL
| >8GF2_A|Chain A, F[auth B]|Spike protein S1|Severe acute respiratory syndrome coronavirus 2 (2697049) RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFSGHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: TYR27 ILE30 TRP31 TYR32 TRP33 GLY53 ASP54 GLY55 GLU56 ARG58 GLY96 GLY97 ILE98 SER99 ASP101 L: SER27 ILE28 ASN29 LYS30 TYR32 TYR49 TRP50 A: TYR369 ASN370 SER371 ALA372 PHE374 SER375 THR376 PHE377 LYS378 CYS379 TYR380 GLY381 VAL382 SER383 PRO384 THR385 LYS386 LEU390 PHE392 ARG408 ASP427 ASP428 PHE429 THR430 PHE515 LEU517 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
